Search for dissertations about: "Escherichia coli fed-batch"

Showing result 1 - 5 of 30 swedish dissertations containing the words Escherichia coli fed-batch.

  2. 1. The temperature-limited fed-batch technique for control of Escherichia coli cultures

    Author : Marie Svensson; Sven-Olof Enfors; John Villadsen; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Endotoxin; Escherichia coli; fed-batch technique; glucose-limitation; mathematical model; Bioengineering; Bioteknik;

    Abstract : The objective of this study was to investigate the physiology and productivity in Escherichia colicultures with emphasis on the temperature-limited fed-batch (TLFB) culture. The TLFB techniquecontrols the oxygen consumption rate of the growing culture by a gradually declining temperaturefrom 37-35 °C down to 20-18 °C. READ MORE

  4. 2. Metabolic analysis of shikimic acid producing Escherichia coli

    Author : Louise Johansson; Avdelningen för kemiteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Biokemi; metabolism; Chemical technology and engineering; transcriptome analysis; phosphorus-limitation; carbon-limitation; fed-batch; chemostat; batch; fermentation technology; Escherichia coli; shikimic acid; Biotechnology; Bioteknik; Metabolism; Biochemistry; principal component analysis; 2D-fluorescence spectrophotometry; metabolic flux analysis; carbon-rich;

    Abstract : Shikimic acid production using recombinant E.coli has been developed during the past 15 years due to the increased demand of this product, primarily as a starting material for the anti-influensa medicine Tamiflu?. A detailed metabolic analysis of a shikimic acid producing E. READ MORE

  5. 3. Performance and Scale-up of Escherichia coli Fed-batch Processes- Oxygenation of the Human Growth Hormone Process

    Author : Andreas Castan; KTH; []
    Keywords : E. coli; fed-batch; DO-control; oxygen transfer; cell lysis; DNA release; mixed acid fermentation; formate formation; oxygen enriched air supply; flow cytometry; recombinant human growth hormone rhGH ;

    Abstract : .... READ MORE

  6. 4. Growth rate control of periplasmic product retention in Escherichia coli

    Author : Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Abstract : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. READ MORE

  7. 5. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Author : Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Abstract : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. READ MORE