Search for dissertations about: "Escherichia coli"

Showing result 6 - 10 of 568 swedish dissertations containing the words Escherichia coli.

2. 6. Membrane and secretory protein production in Escherichia coli

   Author : Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

   Abstract : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. READ MORE

4. 7. Metabolic analysis of shikimic acid producing Escherichia coli

   Author : Louise Johansson; Avdelningen för kemiteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Biokemi; metabolism; Chemical technology and engineering; transcriptome analysis; phosphorus-limitation; carbon-limitation; fed-batch; chemostat; batch; fermentation technology; Escherichia coli; shikimic acid; Biotechnology; Bioteknik; Metabolism; Biochemistry; principal component analysis; 2D-fluorescence spectrophotometry; metabolic flux analysis; carbon-rich;

   Abstract : Shikimic acid production using recombinant E.coli has been developed during the past 15 years due to the increased demand of this product, primarily as a starting material for the anti-influensa medicine Tamiflu?. A detailed metabolic analysis of a shikimic acid producing E. READ MORE

5. 8. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

   Author : Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

   Abstract : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. READ MORE

6. 9. Structural Studies of O-Polysaccharides from Diarrhoeal Escherichia coli

   Author : Tara Ali; Göran Widmalm; Andrej Weintraub; Lennart Kenne; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; Lipopolysaccharide; NMR; biological repeating unit; Organic chemistry; Organisk kemi; Organic Chemistry; organisk kemi;

   Abstract : This thesis describes the structural analysis of O-polysaccharides from the Gram-negative bacterium Escherichia coli that is a diarrhoeal pathogen. The Escherichia coli serotypes investigated were O178, O171, O166 and O128. The methods used in these studies were nuclear magnetic resonance spectroscopy and component analysis. READ MORE

7. 10. Probing Control of Glucose Feeding in Escherichia coli Cultivations

   Author : Mats Åkesson; Institutionen för reglerteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Automatiska system; control engineering; robotics; Automation; dissolved oxygen; acetate; glucose feeding; robotteknik; Escherichia coli; Process control; fed-batch cultivation; reglerteknik; Biotechnology; Bioteknik;

   Abstract : Production of many proteins can today be made using genetically modified organisms. One of the most frequently used host organisms is the bacterium Escherichia coli. A difficulty encountered in cultivations of E. coli is the accumulation of the metabolic by-product acetate which inhibits cell growth and production of a desired protein. READ MORE