Search for dissertations about: "Fluorescent DNA base analog"

Found 3 swedish dissertations containing the words Fluorescent DNA base analog.

  2. 1. Fluorescence Probing of DNA Structures in Biology and Nanotechnology

    Author : Peter Sandin; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA; Fluorescent DNA base analog; UV‐Vis spectroscopy; DNA nanostructure; self‐assembly; time‐resolved fluorescence spectroscopy;

    Abstract : Much has been learnt about DNA since its structure was discovered about half a century ago. Scientists everlasting urge to look beyond and deeper into the intricate machinery of life, has continuously pushed technology forward. READ MORE

  4. 2. Thermodynamics of Peptide Nucleic Acid Interactions with DNA

    Author : Tommi Ratilainen; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; thermodynamics; enthalpy-entropy compensation; ionic strength; nucleic acids; fluorescence energy transfer; PNA; calorimetry; sequence-specific binding;

    Abstract : Peptide Nucleic Acid (PNA) is a DNA mimic with the natural nucleobases attached to a charge-neutral pseudopeptide backbone. It has remarkably strong and sequence-specific binding affinity to natural nucleic acids (DNA and RNA), mainly attributed to the lack of charge in the PNA backbone. READ MORE

  5. 3. The Light-up Probe

    Author : Nicke Svanvik; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

    Abstract : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. READ MORE