Search for dissertations about: "Fredrik Uhlén"

Found 5 swedish dissertations containing the words Fredrik Uhlén.

  2. 1. Nanofabrication of Zone Plates for Hard X-Ray Free-Electron Lasers

    Author : Fredrik Uhlén; Ulrich Vogt; Thomas Wilhein; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; X-ray optics; Zone plates; Nanofabrication; X-ray free-electron lasers; Fysik; Physics;

    Abstract : This Thesis describes the development of hard X-ray zone plates intended for focusing radiation at X-ray free-electron lasers (XFELs). XFELs provide unprecedented brightness and zone plates which are put in the intense X-ray beam are at risk of being damaged. READ MORE

  4. 2. Targeted proteomics methods for protein quantification of human cells, tissues and blood

    Author : Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

    Abstract : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. READ MORE

  5. 3. Development of novel affinity enrichment strategies for clinical applications using selected reaction monitoring

    Author : Andreas Hober; Mathias Uhlén; Fredrik Edfors; Björn Forsström; Johan Malmström; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteomics; mass spectrometry; selected reaction monitoring; absolute quantification; antibody validation; precision medicine; Biotechnology; Bioteknologi;

    Abstract : Proteins are key components of any living organism and an essential part of life itself. They can provide cells with structure and perform life-sustaining intracellular reactions. As organisms grow more complex, this task expands even further. READ MORE

  6. 4. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

    Author : David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

    Abstract : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. READ MORE

  7. 5. Computational analyses of biological sequences -applications to antibody-based proteomics and gene family characterization

    Author : Mats Lindskog; Mathias Uhlén; Fredrik Sterky; Andersson Björn; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; antigen; antibodies; antibody-based proteomics; biocomputing; bioinformatics; Bioinformatics; Bioinformatik;

    Abstract : Following the completion of the human genome sequence, post-genomic efforts have shifted the focus towards the analysis of the encoded proteome. Several different systematic proteomics approaches have emerged, for instance, antibody-based proteomics initiatives, where antibodies are used to functionally explore the human proteome. READ MORE