Search for dissertations about: "GDH"

Showing result 1 - 5 of 6 swedish dissertations containing the word GDH.

2. 1. Second generation of Glucose Biosensors : Biofuel cells and Neurotransitters

   Author : NAJAT BEDEN; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; GDH; PDH; GOx; CDH; mediated electron transfer MET; Os-polymer; Man-Os VI tmen; PVPOs II -Chitosan; Au-NPs-MWCNTS; enzymatic biosensors; non-enzymatic sensor; DA-sensor; Multi-analyte sensor; DA; AA; UA;

   Abstract : Different FAD dependent GDH enzymes together with the Os-redox polymer Os(4,4′-dimethyl-2,2′-bipyridine)2(PVI)10Cl]+ and a modified graphite electrode was shown to have distinct selectivity and high turnover rates towards glucose. The results of this study suggest that such electrodes may be applied either for glucose determination or as bioanodes in glucose biofuel cells. READ MORE

4. 2. 2nd and 3rd generation electrodes for biosensors and biofuel cells

   Author : MNadeem Zafar; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; WrbA; pyranose dehydrogenase; cellobiose dehydrogenase; glucose dehydrogenase; direct electron transfer; Os-polymers; mediated electron transfer; single-walled carbon nanotubes; amperometric biosensor; biofuel cell; screen-printed electrodes; flow injection analysis; cascade reaction; bioanode;

   Abstract : The catalytic and electrochemical properties of tryptophan repressor-binding protein (WrbA), pyranose dehydrogenase (PDH), cellobiose dehydrogenase (CDH) and glucose dehydrogenase (GDH) on carbon electrodes were investigated using cyclic voltammetry and flow injection amperometry to construct biosensors and biofuel cell (BFC) anodes. WrbA has a catalytic FMN-containing domain whereas both PDH and GDH have a catalytic FAD-containing domains. READ MORE

5. 3. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis

   Author : Wei Zhang; Bengt Mannervik; Bengt-Harald Jonsson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; azathioprine; directed evolution; semi-rational design; catalytic mechanism; saturation mutagenesis; reduced amino acid alphabets; molecular docking; protein evolution; multivariate data analysis; epistasis; fitness landscape; evolutionary trajectories; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Abstract : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. READ MORE

6. 4. Bioelectrochemical Applications of Reactions Catalyzed by Immobilized Enzymes

   Author : Xiao-Jing Tang; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Acetate Kinase.; NAD NADH recycling; Enzyme Electrodes; Enzyme reactors; Enzyme Immobilization; Electrochemical detection; Flow Injection Analysis; Analytical chemistry; Analytisk kemi;

   Abstract : The sensitivity of biosensors can be increased substantially by incorporation in a substrate recycling scheme. Based on the amplification of NAD+/NADH by the recycling reaction catalyzed by glycerol dehydrogenase (GDH) and diaphorase, NAD+/NADH can be determined amperometrically with around 1000 times higher sensitivity than for the substrate sensing mode. READ MORE

7. 5. Emergency presentation of colon cancer

   Author : Hanna Gunnarsson; Karolinska Institutet; Karolinska Institutet; []
   Keywords : colon cancer;

   Abstract : Colon cancer is the second most common form of non-skin cancer in Sweden for men and women respectively. Up to one fourth of all colon cancer cases are reported to present as emergencies, which is associated with a higher postoperative mortality and a lower 5 year survival, and affects the total survival in the colon cancer group. READ MORE