Search for dissertations about: "Genome editing"

Showing result 1 - 5 of 34 swedish dissertations containing the words Genome editing.

  2. 1. Understanding Cancer Mutations by Genome Editing

    Author : Muhammad Akhtar Ali; Tobias Associate Professor; Thierry Soussi; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genome editing; rAAV; ZBED6; DIP2C; KRAS; BRAF; colorectal cancer; tumor driver genes; cancer pathways;

    Abstract : Mutational analyses of cancer genomes have identified novel candidate cancer genes with hitherto unknown function in cancer. To enable phenotyping of mutations in such genes, we have developed a scalable technology for gene knock-in and knock-out in human somatic cells based on recombination-mediated construct generation and a computational tool to design gene targeting constructs. READ MORE

  4. 2. Improving integration efficiency and precision of CRISPR/Cas9-mediated genome editing

    Author : Sandra Wimberger; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Genome editing; CRISPR-Cas9; DNA repair; genomic integration; off-targets;

    Abstract : The clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR-associated protein 9 (Cas9) system has revolutionized the field of genome engineering, providing a cost-effective and fast tool for targeted gene modifications. Endogenous repair pathways, including error-prone non-homologous end joining and alternative end joining, or precise homology-directed repair (HDR), mend Cas9-induced DNA double-strand breaks. READ MORE

  5. 3. Investigating the molecular mechanism of photoprotection, qH, in Arabidopsis thaliana

    Author : Pierrick Bru; Alizée Malnoë; Stefano Caffarri; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Photosynthesis; Photoprotection; non photochemical quenching; qH; genetic screen; genome editing; biology; biologi; Biochemistry; biokemi; molekylärbiologi; Molecular Biology;

    Abstract : Photoprotection mechanisms in plants play a crucial role in maintaining photosystem integrity and preventing photooxidative damage. In this study, we aimed to identify new molecular players involved in the qH process, a sustained photoprotective mechanism. READ MORE

  6. 4. Genome editing of oilseed species by CRISPR/Cas9 for trait improvement

    Author : Sjur Sandgrind; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

    Abstract : The demand for vegetable oils for food and other applications is rapidly increasing due to a growing population and an urgent need to replace fossil oils. Furthermore, there are several challenges facing agricultural production, such as a shortage of arable land, climate change, and water pollution due to nutrient leaching from extensive use of fertilizers and tillage. READ MORE

  7. 5. Targeting allelic loss in colorectal cancer

    Author : Verónica Rendo; Tobias Sjöblom; Nickolas Papadopoulos; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; colorectal cancer; gene editing; loss of heterozygosity; targeted therapy; whole genome sequencing;

    Abstract : Targeted cancer therapy exploits molecular differences between tumor and normal cells to selectively kill cancer cells. Whereas targeting of activated oncogenes has proved clinically useful, few current therapies exploit loss-of-function mutations in tumor suppressor genes or in the genome at large. READ MORE