Search for dissertations about: "Hans Hebert"

Showing result 1 - 5 of 6 swedish dissertations containing the words Hans Hebert.

  2. 1. Protein structure dynamics and interplay : by single-particle electron microscopy

    Author : Hans Elmlund; Hans Hebert; José Maria Carazo; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; biophysics; biochemistry; molecular; Biophysical chemistry; Biofysikalisk kemi;

    Abstract : Single-particle cryo-electron microscopy (cryo-EM) is a method capable of obtaining information about the structural organization and dynamics of large macromolecular assemblies. In the late nineties, the method was suggested to have the potential of generating “atomic resolution” reconstructions of particles above a certain mass. READ MORE

  4. 2. Single-particle cryo-electron microscopy of macromolecular assemblies

    Author : Kimberley Cheng; Hans Hebert; Roland Beckmann; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Abstract : In this thesis, single-particle cryo-electron microscopy (cryo-EM) was used to study the structure of three macromolecular assemblies: the two hemocyanin isoforms from Rapana thomasiana, the Pyrococcus furiosus chaperonin, and the ribosome from Escherichia coli. Hemocyanins are large respiratory proteins in arthropods and molluscs. READ MORE

  5. 3. Towards unbiased 3D reconstruction : in single-particle cryo-electron microscopy

    Author : Dominika Elmlund; Hans Hebert; Sarah Butcher; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Abstract : Cryo-electron microscopy of freestanding molecules (single-particles) plays a pivotal role in the difficult and pressing challenge of determining the structures of large macromolecular complexes. Molecular volumes are generated by aligning large sets of randomly oriented two-dimensional (2D) projection images in three dimensions (3D) before reconstruction is performed using tomographic techniques. READ MORE

  6. 4. Structural studies of microbubbles and molecular chaperones using transmission electron microscopy

    Author : Johan Härmark; Philip JB Köck; Hans Hebert; Reinhard Rachel; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transmission electron microscopy; Contrast agent; Microbubble; Polyvinyl alcohol; Single particle analysis; Heat shock protein; Molecular chaperone; Technology and Health; Teknik och hälsa;

    Abstract : Ultrasound contrast agents (CAs) are typically used in clinic for perfusion studies (blood flow through a specific region) and border delineating (differentiate borders between tissue structures) during cardiac imaging. The CAs used during ultrasound imaging usually consist of gas filled microbubbles (MBs) (diameter 1-5 μm) that are injected intravenously into the circulatory system. READ MORE

  7. 5. Structural studies of membrane proteins using transmission electron microscopy

    Author : Qie Kuang; Hans Hebert; Catherine Vénien-Bryan; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : Membrane proteins play important roles for living cells. They control transportation of ions, solutes, and nutrients across the membrane and catalyze metabolic reactions. Transmission electron microscopy has its advantages in convenient sample preparation, straightforward structural determination, and wide applications for diverse specimens. READ MORE