Search for dissertations about: "Human proteome"

Showing result 1 - 5 of 107 swedish dissertations containing the words Human proteome.

  1. 1. Mapping the human proteome using bioinformatic methods

    Author : Linn Fagerberg; Mathias Uhlén; Alvis Brazma; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; proteome; transcriptome; bioinformatics; membrane protein prediction; subcellular localization; protein expression level; cell line; immunohistochemistry; immunofluorescence; Bioinformatics; Bioinformatik; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : The fundamental goal of proteomics is to gain an understanding of the expression and function of the proteome on the level of individual proteins, on the level of defined cell types and on the level of the entire organism. In this thesis, the human proteome is explored using membrane protein topology prediction methods to define the human membrane proteome and by global protein expression profiling, which relies on a complex study of the location and expression levels of proteins in tissues and cells. READ MORE

  2. 2. Antibody-based subcellular localization of the human proteome

    Author : Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Biotechnology; Bioteknologi;

    Abstract : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. READ MORE

  3. 3. The Membrane Proteome : Evolution, Characteristics and Classification

    Author : Markus Sällman Almén; Helgi Schiöth; Robert Fredriksson; Marc Robinson-Rechavi; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Membrane proteome; molecular evolution; GPCRs; Dispanins; IFITM; Medicinsk vetenskap; Medical Science;

    Abstract : Membrane proteins are found in all kingdoms of life and are essential for cellular interactions with the environment. Although a large research effort have been put into this group many membrane proteins remains uncharacterized, both in terms of function and evolutionary history. READ MORE

  4. 4. Spatial proteome profiling of the compartments of the human cell using an antibody-based approach

    Author : Mikaela Wiking; Emma Lundberg; Björn Önfelt; Torbjörn Gräslund; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Fluorescence imaging; Human proteome; Human Protein Atlas; Immunofluorescence; Mitochondria; Organelles; Spatial distribution; Biotechnology; Bioteknologi;

    Abstract : The human cell is complex, with countless processes ongoing in parallel in specialized compartments, the organelles. Cells can be studied in vitro by using immortalized cell lines that represent cells in vivo to a varying degree. READ MORE

  5. 5. Spatiotemporal characterization of the human proteome

    Author : Diana Mahdessian; Emma Lundberg; Arne Lindqvist; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Spatial proteomics; Spatiotemporal proteomics; Immunofluorescence; Human Protein Atlas; Cell compartments; Single cell proteomics; Cell cycle; Cancer;

    Abstract : Characterizing the molecular components of the basic unit of life; the cell, is crucial for a complete understanding of human biology. The cell is divided into compartments to create a suitable environment for the resident proteins to fulfill their functions. READ MORE