Search for dissertations about: "Hydrophobic interaction chromatography"
Showing result 6 - 10 of 27 swedish dissertations containing the words Hydrophobic interaction chromatography.
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6. Modeling and Optimization of Reversed-Phase Chromatography : Effects of Modulators and Temperature
Abstract : Many widespread diseases, such as diabetes, various types of cancer, and aggressive versions of influenza, are treated or prevented with biopharmaceuticals. Biopharmaceuticals are drugs that are based on proteins, peptides, antibodies, attenuated viruses (vaccines), and other biomolecules that are synthesized predominately in bacteria, yeast, and mammalian cells. READ MORE
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7. Superporous agarose a new material for chromatography
Abstract : Agarose, the most commonly employed chromatography base material in industrial purification processes for biomolecules – proteins, has almost ideal properties. The major disadvantage of the agarose support is its poor mechanical strength which excludes its use in high speed separations where small particles are needed in order to achieve improved chromatographic efficiency. READ MORE
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8. Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein
Abstract : This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. READ MORE
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9. HYDROPHOBIC PEPTIDE TAGS - As tools in bioseparation and investigation of recombinant proteins
Abstract : This thesis describes the possibility of using hydrophobic peptide tags as tools for bioseparation and for the investigation of recombinant proteins. The hydrophobicity of a protein can easily be changed by minor modifications, such as fusions of tyrosine-containing peptides. READ MORE
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10. A Model Based Approach to Robustness Analysis and Optimisation of Chromatography Processes
Abstract : One of the major issues in the pharmaceutical industry today is the cost of development and validation of the production of the active pharmaceutical ingredients. The development, optimization and validation of a production plant have been dominated by an extensive experimental approach. READ MORE