Search for dissertations about: "IMAC"

Showing result 1 - 5 of 14 swedish dissertations containing the word IMAC.

  2. 1. Polymer Displacement and Supermacroporous Cryogels - Two New Tools in Chromatographic Separation of Biomolecules and Bioparticles

    Author : Pär Arvidsson; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biopolymers; Polymerteknik; Biochemical technology; Polymer technology; IMAC; dye-affinity; affinity chromatography; viable cells; cell chromatography; non-clarified cell homogenate; cryogel; Polymer displacement; supermacroporous; Biokemisk teknik;

    Abstract : Production of protein is often accompanied with difficulties due to the large amounts of different biological material resulting from fermentation. This makes purification necessary and affinity chromatography is usually involved as being biospecific, although suffering from limitations as low recovery due to ineffective elution and inability to handle particulate materials. READ MORE

  4. 2. Membrane Protein Tailoring and Structural Studies of Leukotriene C4 Synthase

    Author : Daniel Martinez Molina; Pär Nordlund; Robert M. Stroud; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; membrane proteins; random mutagenesis; detergent screening; Leukotriene C₄ synthase; IMAC; Structural biology; Strukturbiologi; Biochemistry; biokemi;

    Abstract : Despite a dramatic increase in the number of proteins that have been structurally characterized in recent years, there are still less than 200 unique structures of membrane proteins known today. This is only 1% of the total number of unique protein structures found in structural databases worldwide. READ MORE

  5. 3. High-throughput protein analysis using mass spectrometry-based methods

    Author : Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Keywords : Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Abstract : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. READ MORE

  6. 4. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Author : Florent Bernaudat; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE

  7. 5. Sample preparation and mass spectrometry in proteome studies

    Author : Daniel Hirschberg; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Proteome studies aim to link gene to function. Instead of screening for function in a specific protein as in classical protein chemistry, proteins are now screened against a specific phenotype, disease or metabolic state. READ MORE