Search for dissertations about: "Institutionen för naturvetenskaplig biokemi"

Showing result 1 - 5 of 90 swedish dissertations containing the words Institutionen för naturvetenskaplig biokemi.

  2. 1. Structural studies on the integral membrane protein, ubiquinol oxidase from Escherichia coli

    Author : Jeff Abramson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; crystal structure; ubiquinol oxidase; membrane protein; oxidases; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Heme-copper oxidases are redox-driven proton pumps that couple the reduction of molecular oxygen to water with the vectorial translocation of protons across the membrane. The proton gradient generated by heme-copper oxidases and the other members of the aerobic respiratory chain is ultimately used to drive the synthesis of ATP. READ MORE

  4. 2. Partition chromatography of drugs on immobilized liposomes and biomembranes : A method applicable to screening in drug design

    Author : Farideh Beigi; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : One of the main pathways for drug passage into cells is diffusion across the lipid bilayer ofthe cell membrane. A key factor in this process is the drug partitioning into the bilayer, whichcan be translated into a retention in a chromatographic system. READ MORE

  5. 3. Multiple Functions of Glutathione Transferases : A Study on Enzymatic Function, Regulatory Role and Distribution in Mouse and Man

    Author : Maryam Edalat; Joseph De Pierre; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : To cope with various endogenous toxin and xenobiotics nature has equipped the organisms with a proper protection system. Glutathione transferases (GSTs) are important components of the cellular defense against oxidative stress. These proteins appear to be suited for different tasks. READ MORE

  6. 4. Electrochromatography and liquid chromatography : Microtechniques, instrumentation and applications

    Author : Christer Ericson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : The thesis focuses on the design of efficient chromatographic and electrochromatographic microcolumns where a continuous bed is polymerized in situ in a capillary or a channel in a microchip device. In our technique, a solution of selected monomers is simply sucked or pressed into the capillary or microchip channel for subsequent polymerization. READ MORE

  7. 5. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Author : Lars O. Hansson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. READ MORE