Search for dissertations about: "Isoenzymes : analysis"

Showing result 1 - 5 of 8 swedish dissertations containing the words Isoenzymes : analysis.

  2. 1. Cellulose hydrolysis by Trichoderma reesei cellulases: Studies on adsorption, sugar production and synergism of cellobiohydrolase I, II and endoglucanase II

    Author : Jozsef Medve; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; FPLC; cellulose hydrolysis; Trichoderma reesei; cellulase; cellobiohydrolase; endoglucanase; adsorption isotherm; synergism; enzyme purification; isoenzymes; quantitative analysis; Biochemistry; Metabolism; Biokemi;

    Abstract : Three major cellulases, cellobiohydrolase I and II (CBH I and II) and endoglucanase II (EG II) of Trichoderma reesei have been purified by ion-exchange chromatography in an FPLC system. Microcrystalline cellulose (Avicel) was hydrolysed by the single enzymes and by equimolar mixtures of CBH I-CBH II and CBH I-EG II. READ MORE

  4. 2. Development of bioluminescent reagents for kinetic analysis and some applications in clinical chemistry

    Author : Inger Styrélius; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : Firefly luciferase and a bacterial luciferase system have been used to develop analytical methods suitable for use in clinical analysis.The influence of the level of NADH:FMN oxidoreductase on the kinetics of light emission in the bacterial luciferase system was studied. READ MORE

  5. 3. Human α2,3-sialyltransferases. Structure and Function of Alternatively Spliced Transcripts

    Author : Ammi Grahn; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; a2; 3-sialyltransferase; isotranscript; isoenzyme; alternative splicing; capillary electrophoresis;

    Abstract : The recruitment of human peripheral blood leukocytes to sites of infection and inflammation requires the surface expression of Sialyl Lewis x glycoconjugates (SLex) on white blood cells and their interaction with E- and P-selectins on activated endothelial cells. Human ST3Gal III, ST3Gal IV and ST3Gal VI genes code for a2,3?sialyltransferases potentially involved in the biosynthesis of the SLex epitope. READ MORE

  6. 4. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Author : Lars O. Hansson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. READ MORE

  7. 5. Discovery of fiber-active enzymes in Populus wood

    Author : Henrik Aspeborg; KTH; []
    Keywords : Populus; xylogenesis; secondary cell wall; cellulose; hemicellulose; microarrays; transcript profiling; carbohydrate-active enzyme; glycosyltransferase; glycoside hydrolase;

    Abstract : Renewable fibers produced by forest trees provide excellentraw material of high economic value for industrialapplications. Despite this, the genes and corresponding enzymesinvolved in wood fiber biosynthesis in trees are poorlycharacterized. READ MORE