Search for dissertations about: "Isoenzymes"

Showing result 21 - 25 of 36 swedish dissertations containing the word Isoenzymes.

  2. 21. Catalysis and Site-Specific Modification of Glutathione Transferases Enabled by Rational Design

    Author : Sofia Håkansson Hederos; Lars Baltzer; Kerstin Broo; Linköpings universitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Catalysis; Glutathione Transferases; Rational design; Protein design; Site-specific modification; thiolester hydrolysis; Enzyme engineering; Enzymteknik;

    Abstract : This thesis describes the rational design of a novel enzyme, a thiolester hydrolase, derived from human glutathione transferase (GST) A1-1 by the introduction of a single histidine residue. The first section of the thesis describes the design and the determination of the reaction mechanism. READ MORE

  4. 22. Cytochrome P450 2C9, 2C19 and 2D6 genetic polymorphisms : evaluation of genotyping as a tool for individualised treatment

    Author : Maria Gabriella Scordo; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Drug metabolism; CYP2C9; CYP2C19; CYP2D6; genotyping; polymorphism; risperidone; warfarin; fluoxetine; phenytoin; extrapyramidal side-effects;

    Abstract : Recent advances in the understanding of drug metabolism and the molecular biology of CYPs have generated great expectations regarding the use of pharmacogenetic testing as a tool to individualize drug treatment. The individual pharmacogenetic constitution might in principle predict the response to drugs, hormones and toxins. READ MORE

  5. 23. A Novel Route for Construction of Multipurpose Receptors through Chemical Modification of Glutathione Transferases

    Author : Johan Viljanen; Bengt Harald Jonsson; Kerstin Broo; Sophia Hober; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Human GST A1-1; site-specific covalent modification; tyrosine 9; lysine 216; methionine 208; multipurpose receptor; pattern recognition; protein purification; Chemistry; Kemi;

    Abstract : This thesis describes how the human Alpha class glutathione transferase (GST) A1-1 can be reprogrammed either to function as a multipurpose biosensor for detection of small molecule analytes, or as a handle providing for more efficient protein purification.A novel, user-friendly, and efficient method for site-specific introduction of functional groups into the active site of hGST A1-1 is the platform for these achievements. READ MORE

  6. 24. Experimental and computational investigation of affinity and selectivity factors in CYP2D6 and CYP3A4 mediated metabolism

    Author : Britta Bonn; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cytochrome P450; in vitro metabolism; biotransformation; computational modeling; drug design;

    Abstract : The assessment of ADME properties and metabolic behavior of a drug is central in drug discovery and drug design. The main target for studies of metabolic properties is the Cytochrome P450 (CYP), which is responsible for the metabolism of a majority of drugs on the market and consequently also involved in many drug-drug interactions. READ MORE

  7. 25. Cyanobacteria in symbiosis with plants : Protein patterns and regulatory mechanisms

    Author : Martin Ekman; Birgitta Bergman; Dave G. Adams; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cyanobacteria; symbiosis; Nostoc; Gunnera; Azolla; nitrogenase; modification; heterotrophic; proteomics; unsequenced; Plant physiology; Växtfysiologi;

    Abstract : Cyanobacteria belonging to the heterocystous genus Nostoc, capable of oxygenic photosynthesis and nitrogen fixation via the enzyme nitrogenase, may form symbiotic associations with plants. In these associations most of the N2 fixed by the symbiotic cyanobacteria (cyanobiont) is transferred to the host, which in turn supplies the cyanobiont with fixed carbon. READ MORE