Search for dissertations about: "Jan-Willem de Gier"

Showing result 1 - 5 of 13 swedish dissertations containing the words Jan-Willem de Gier.

  2. 1. Functional aspects of inorganic phosphate transport

    Author : Michael R. Andersson; Bengt Persson; Jan Willem de Gier; Linnéuniversitetet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; phosphate membrane transport mutagenesis saccharomyces cerevisae; Biokemi; Biochemistry;

    Abstract : Inorganic phosphate is an essential nutrient for all organisms. It is required for many cellular components as nucleic acids and phospholipids, and as energy-carrying compounds such as ATP. Thus, a regulated uptake of this pivotal nutrient is of outermost importance. READ MORE

  4. 2. Protein targeting, translocation and insertion in Escherichia coli : Proteomic analysis of substrate-pathway relationships

    Author : Louise Baars; Jan-Willem de Gier; Jan-Maarten van Dijl; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein biogenesis; targeting; translocation; insertion; SecB; SecE; E. coli; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Approximately 10% of the open reading frames in the genome of the Gram-negative bacterium E. coli encodes secretory proteins, and 20% encodes integral inner membrane proteins (IMPs). READ MORE

  5. 3. Protein production in the E. coli cell envelope

    Author : Thomas Baumgarten; Jan-Willem de Gier; Dirk-Jan Scheffers; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; Biochemistry; biokemi;

    Abstract : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. READ MORE

  6. 4. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli

    Author : David Drew; Jan-Willem de Gier; Gunnar von Heijne; Bert Poolman; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; GFP; membrane protein; topology; overexpression; high-throughput; Biochemistry; Biokemi;

    Abstract : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. READ MORE

  7. 5. Production and folding of proteins in the periplasm of Escherichia coli

    Author : Rageia Elfageih; Jan-Willem de Gier; Hans-Georg Koch; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; periplasm; recombinant protein production; disulfide bond containing proteins; translation initiation region; protein translocation machinery; co-translational folding; neuraminidases; biokemi; Biochemistry;

    Abstract : The Gram-negative bacterium E. coli is the most widely used host for the production of recombinant proteins. Disulfide bond containing recombinant proteins are usually produced in the periplasm of E. coli since in this compartment of the cell - in contrast to the cytoplasm - disulfide bond formation is promoted. READ MORE