Search for dissertations about: "Karl Hult"

Showing result 6 - 10 of 15 swedish dissertations containing the words Karl Hult.

  2. 6. Serine Hydrolase Selectivity : Kinetics and applications in organic and analytical chemistry

    Author : Anders Hamberg; Karl Hult; Dick Janssen; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; monoacylation of diols; kinetic resolution; thermodynamics in enzyme catalysis; enzyme engineering; Biochemistry; Biokemi;

    Abstract : The substrate selectivities for different serine hydrolases were utilized in various applications, presented in papers I-VI. The articles are discussed in the thesis in view of the kinetics of the enzyme catalysis involved. READ MORE

  4. 7. Lipase chemoselectivity - kinetics and applications

    Author : Cecilia Hedfors; Karl Hult; Mats Martinelle; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; Rhizomucor miehei lipase; active site titration; kinetics; chemoselectivity; thiol; alcohol; thiol-functionalized polyesters; Biochemistry; Biokemi;

    Abstract :   A chemoselective catalyst is preferred in a chemical reaction where protecting groups otherwise are needed. The two lipases Candida antarctica lipase B and Rhizomucor miehei lipase showed large chemoselectivity ratios, defined as (kcat/KM)OH / (kcat/KM)SH, in a transacylation reaction with ethyl octanoate as acyl donor and hexanol or hexanethiol as acyl acceptor (paper I). READ MORE

  5. 8. Lipase selectivity in functional polyester synthesis

    Author : Cecilia Hedfors; Karl Hult; Romas Kazlauskas; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi;

    Abstract : Enzyme selectivity means that the enzyme´s preferences towards competing substrates will be different. In this thesis, the enzyme selectivity has been studied for utilization in synthesis of functionalized macromonomers. READ MORE

  6. 9. Lipase-Lipid Interactions Studied by Site-Directed Labeling and Biological Spectroscopy

    Author : Eva Hedin; Karl Hult; Marek Brzozowski; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; thermomyces lanuginous lipase; site-directed labeling; lipase-lipid interactions; Biochemistry; Biokemi;

    Abstract : This thesis describes the study of lipase–lipid interactions of the triglyceride lipase Thermomyces lanuginosus lipase, TLL, by use of site-directed chemical labeling and biological spectroscopy. Several single-cysteine variants of TLL were produced, and labeled with probes containing either an affinity-, a fluorescence- or a spin-label functionality. READ MORE

  7. 10. Rational redesign of Candida antarctica lipase B

    Author : Anders Magnusson; Karl Hult; Bernhard Hauer; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; rational redesign; secondary alcohols; substrate-assisted catalysis; S-selective; entropy; aldolase; stereospecificity pocket; oxyanion hole.; Biokemi; Biochemistry; Biokemi;

    Abstract : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. READ MORE