Search for dissertations about: "Lisha Wu"

Found 2 swedish dissertations containing the words Lisha Wu.

  2. 1. NMR structral studies on ruthenium complexes binding to DNA

    Author : Lisha Wu; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NMR; DNA binding; Ru-complex dimer; CD; fluorescence; molecular dynamics simulation;

    Abstract : The Ru-complex Λ,Λ-[bidppz(bipy)4Ru2]4+ (Λ,Λ-B) exhibits multiple modes of interactions with the palindromic DNA dodecamer [d(CGCGAATTCGCG)]2, however excluding any type of intercalation as shown by both optical spectroscopy and NMR. NMR observation of Λ,Λ-B titration into a DNA solution results in both a transient binding, with observation of fast exchange for protons located near the ends of the DNA duplex, and a more stable interaction in the DNA minor groove indicated by a slow exchange mechanism and binding sites for G2, G4 and A5 of the same DNA strand. READ MORE

  4. 2. Unveiling biomacromolecule interactions-NMR and optical spectroscopy studies on ligand binding to DNA and lysozyme

    Author : Lisha Wu; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; ruthenium compounds; intercalation; Hoechst 33258; CD; fluorescence; DNA; structure rearrangements.; lysozyme; NMR;

    Abstract : Studies on intercalation of bulky binuclear ruthenium compounds into DNA have attracted attention due to their slow dissociation rate and sequence selectivity. Previous results showed that dumb-bell shaped molecules of the type [μ-(bidppz)(L)4Ru2]4+, L=phenanthroline or bipyridine, bind rapidly on the surface of DNA prior to intercalation. READ MORE