Search for dissertations about: "MEMBRANE-PROTEIN"

Showing result 6 - 10 of 313 swedish dissertations containing the word MEMBRANE-PROTEIN.

  2. 6. Marginally hydrophobic transmembrane α-helices shaping membrane protein folding

    Author : Tuuli Minttu Virkki de Marothy; Arne Elofsson; William Dowhan; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; membrane protein folding; hydrophobicity; translocon; transmembrane helix; marginally hydrophobic transmembrane helices; orientational preference; positive inside rule; aquaporin 1; Biochemistry; biokemi;

    Abstract : Most membrane proteins are inserted into the membrane co-translationally utilizing the translocon, which allows a sufficiently long and hydrophobic stretch of amino acids to partition into the membrane. However, X-ray structures of membrane proteins have revealed that some transmembrane helices (TMHs) are surprisingly hydrophilic. READ MORE

  4. 7. Structural modeling of membrane transporter proteins

    Author : Jonas Almqvist; Sven Hovmöller; David van der Spoel; Stockholms universitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; membrane protein structure; membrane protein crystallography; membrane protein structure modelling; Structural biochemistry; Strukturbiokemi; strukturkemi; Structural Chemistry;

    Abstract : A fundamental process of all living organisms - the transport of molecules across cellular membranes through membrane transport proteins - is investigated.After a brief review of general properties of biological membranes follows a recollection of the major methods of membrane transport that Nature utilizes (Chapter 1). READ MORE

  5. 8. Structural studies on the integral membrane protein, ubiquinol oxidase from Escherichia coli

    Author : Jeff Abramson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; crystal structure; ubiquinol oxidase; membrane protein; oxidases; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Heme-copper oxidases are redox-driven proton pumps that couple the reduction of molecular oxygen to water with the vectorial translocation of protons across the membrane. The proton gradient generated by heme-copper oxidases and the other members of the aerobic respiratory chain is ultimately used to drive the synthesis of ATP. READ MORE

  6. 9. Membrane protein proteomics - Novel method for membrane protein identification and quantification

    Author : Maria Bentz; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemisk teknik; mebran; steroider; Lipider; membranes; steroids; Lipids; enzymologi; Proteiner; Membrane proteins; Proteomics; Proteinase K; N-terminal labelling; Relative Quantitation; Database search; Cancer; Cytology; oncology; cancerology; Cytologi; onkologi; cancer; enzymology; Proteins; Biochemical technology;

    Abstract : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. READ MORE

  7. 10. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

    Author : Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

    Abstract : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. READ MORE