Search for dissertations about: "Mathias Uhlén"

Showing result 6 - 10 of 22 swedish dissertations containing the words Mathias Uhlén.

  2. 6. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses

    Author : Spyros Darmanis; Masood Kamali-Moghaddam; Ulf Landegren; Mathias Uhlen; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. READ MORE

  4. 7. Targeted proteomics methods for protein quantification of human cells, tissues and blood

    Author : Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

    Abstract : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. READ MORE

  5. 8. Mapping the human proteome using bioinformatic methods

    Author : Linn Fagerberg; Mathias Uhlén; Alvis Brazma; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteome; transcriptome; bioinformatics; membrane protein prediction; subcellular localization; protein expression level; cell line; immunohistochemistry; immunofluorescence; Bioinformatics; Bioinformatik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Abstract : The fundamental goal of proteomics is to gain an understanding of the expression and function of the proteome on the level of individual proteins, on the level of defined cell types and on the level of the entire organism. In this thesis, the human proteome is explored using membrane protein topology prediction methods to define the human membrane proteome and by global protein expression profiling, which relies on a complex study of the location and expression levels of proteins in tissues and cells. READ MORE

  6. 9. Exploring genetic heterogeneity in cancer using high-throughput DNA and RNA sequencing

    Author : Erik Fasterius; Cristina Al-Khalili Szigyarto; Mathias Uhlén; Peter-Bram ’t Hoen; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Biotechnology; bioinformatics; RNA-seq; WGS; WES; systems biology; variant analysis; single nucleotide variant; gene expression; machine learning; clustering; open source; R; bioconductor; Python; Bioteknologi; Biotechnology;

    Abstract : High-throughput sequencing (HTS) technology has revolutionised the biomedical sciences, where it is used to analyse the genetic makeup and gene expression patterns of both primary patient tissue samples and models cultivated in vitro. This makes it especially useful for research on cancer, a disease that is characterised by its deadliness and genetic heterogeneity. READ MORE

  7. 10. Characterization of antibody specificity using peptide array technologies

    Author : Björn Forsström; Mathias Uhlén; Markus Templin; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; Epitope mapping; Peptide array; Suspension bead array; Antigen; Specificity; Cross-reactivity; Immunization; Immunogenicity; Bioteknologi; Biotechnology;

    Abstract : Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. READ MORE