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Showing result 1 - 5 of 55 swedish dissertations matching the above criteria.

2. 1. Matrix-assisted laser desorption and ionization of biomolecules

   Author : Ann Westman; Uppsala universitet; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP;

   Abstract : .... READ MORE

4. 2. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

   Author : Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

   Abstract : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. READ MORE

5. 3. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

   Author : Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

   Abstract : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. READ MORE

6. 4. Novel clean-up, concentration and laser desorption/ionization strategies for mass spectrometry

   Author : Mohammadreza Shariatgorji; Leopold Ilag; Juan Astorga-wells; Bernhard Spengler; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; ESI-MS; SALDI-MS; Electrocapture; Sample treatment; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

   Abstract : Despite valuable advantages of mass spectrometry (MS) techniques they still have drawbacks that need to be overcome, notably their sensitivity to various kinds of interfering substances and associated difficulties involved in detecting signals from analytes present in trace quantities. Thus, high quality analytical data could be acquired by improving sample pre-treatment techniques and/or ionization methods to increase ionization yields and/or avoid the generation of unwanted ions. READ MORE

7. 5. Tools for applied soft ionization mass spectrometry

   Author : Joakim Romson; Åsa Emmer; Peter Dinér; Steven Wilson; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; Electrospray ionization; Matrix assisted laser desorption ionization; Mass accuracy; Mass shifts; Ion trap; Capillary electrophoresis; Sample concentration; Masspektrometri; Elektrospray-jonisering; Matrisassisterad laser desorbtion jonisering; Mass-exakthet; Masskift; Jonfälla; Kapillärelektrofores; Provkoncentration; Kemi; Chemistry;

   Abstract :  Mass spectrometry (MS) is an important analytical tool. Its most importantadvantages are sensitivity, resolution, and accurate and precise m/zmeasurements. To get the best results from an instrument, the user musthave knowledge of its limitation and requirements. READ MORE