Search for dissertations about: "Meta Kuehn"

Found 1 swedish dissertation containing the words Meta Kuehn.

2. 1. Intracellular vesicles induced by monotopic membrane protein in Escherichia coli

   Author : Hanna M. Eriksson; Åke Wieslander; Meta Kuehn; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; intracellular vesicles; Escherichia coli; glycosyltransferase; overexpression; optimization; detergent; screening; lipid composition; Biochemistry; Biokemi; Biochemistry; biokemi;

   Abstract : The monotopic membrane protein alMGS, a glycosyltransferase catalyzing glucolipid synthesis in Acholeplasma laidlawii, was overexpressed in Escherichia coli. Optimization of basic growth parameters was performed, and a novel method for detergent and buffer screening using a small size-exclusion chromatography was developed. READ MORE