Search for dissertations about: "Mikael Widersten"

Showing result 1 - 5 of 21 swedish dissertations containing the words Mikael Widersten.

  1. 1. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

    Author : Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

    Abstract : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. READ MORE

  2. 2. Glutathione transferases : studies on the structure and function of recombinant enzymes

    Author : Mikael Widersten; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP;

    Abstract : .... READ MORE

  3. 3. Carboligation using the aldol reaction : A comparison of stereoselectivity and methods

    Author : Derar Al-Smadi; Mikael Widersten; Ulf Nilsson; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; aldol reaction; cinchonine; FSA enzyme; homologation reactions; phenylacetaldehyde derivatives; samarium diiodide.;

    Abstract : The research summarized in this thesis focuses on synthesizing aldehyde and aldol compounds as substrates and products for the enzyme D-fructose-6-aldolase (FSA). Aldolases are important enzymes for the formation of carbon-carbon bonds in nature. READ MORE

  4. 4. Computational modelling of enzyme selectivity

    Author : Paul Bauer; Shina Caroline Lynn Kamerlin; Mikael Widersten; Kenneth Ruud; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; enantiomer; epoxide hydrolase; chiral catalysis; empirical valence bond approach; method development; Biokemi; Biochemistry;

    Abstract : Enantioselective reactions are one of the ways to produce pure chiral compounds. Understanding the basis of this selectivity makes it possible to guide enzyme design towards more efficient catalysts. READ MORE

  5. 5. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

    Author : Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

    Abstract : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. READ MORE