Search for dissertations about: "Mild elution"

Found 5 swedish dissertations containing the words Mild elution.

  2. 1. Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

    Author : Julia Scheffel; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibodies; Antibody fragments; Biomanufacturing; Protein purification; Protein engineering; Protein A; ZCa; Calcium; Mild elution; Bioteknologi; Biotechnology;

    Abstract : Antibodies are essential proteins in both our bodies and biotechnological research, and hold outstanding therapeutic value. The market for antibody-based therapeutics has grown exponentially during the last decades, owing to several advantages over small molecule drugs, such as fewer undesirable side effects associated with a higher target specificity. READ MORE

  4. 2. Strategies for de novo DNA sequencing

    Author : Anna Blomstergren; KTH; []
    Keywords : DNA sequencing; DNA purification; automation; solid-phase; streptavidin; biotin; modular probes; Helicobacter pylori; cag PAI;

    Abstract : The development of improved sequencing technologies hasenabled the field of genomics to evolve. Handling andsequencing of large numbers of samples require an increasedlevel of automation in order to obtain high throughput andconsistent quality. READ MORE

  5. 3. Engineering of small IgG binding domains for antibody labelling and purification

    Author : Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Abstract : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. READ MORE

  6. 4. Carbohydrate-specific, boronate-containing copolymers: from sugar-sensing to cell adhesion

    Author : Marina Kuzimenkova; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface grafting; chemisorption; boronic acid; carbohydrates; affinity; chromatography; smart polymers; biomimetics; hydrogels; molecular recognition; diffusion; sugar-sensing; cell adhesion;

    Abstract : Carbohydrates play a major role in many cell recognition events and metabolic pathways of living organisms. Synthetic carbohydrate receptors are of great interest for the chemical design of sugar sensors, materials for cell separation, cell immobilization, drug delivery and other biomedical/biotechnological applications. READ MORE

  7. 5. Engineering alternative scaffold proteins for conditional targeting

    Author : Malin Jönsson; Sophia Hober; Mark Howarth; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Conditional targeting; Calcium; Biotechnology; Bioteknologi;

    Abstract : Engineering naturally occurring proteins enables us to customize affinity domains according to our specific needs, tailoring them to become an important tool in a wide array of applications limited merely by our creativity. One of nature’s ways to regulate protein activity is by creating a functional change through alteration of a protein’s tertiary structure upon interaction with metal ions. READ MORE