Search for dissertations about: "Molekylär bioteknik"

Showing result 1 - 5 of 61 swedish dissertations containing the words Molekylär bioteknik.

  2. 1. Engineering of Affibody molecules for Radionuclide Molecular Imaging and Intracellular Targeting

    Author : Camilla Hofström; Torbjörn Gräslund; Serge Muyldermans; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; Affibody molecules; affinity proteins; radionuclide molecular imaging; intracellular targeting; EGFR; HER2; IGF-1R; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : Affibody molecules are small (7 kDa) affinity proteins of non-immunoglobulin origin that have been generated to specifically interact with a large number of clinically important molecular targets.In this thesis, Affibody molecules have been employed as tracers for radionuclide molecular imaging of HER2- and IGF-1R-expressing tumors, paper I-IV, and for surface knock-down of EGFR, paper V. READ MORE

  4. 2. An albumin-binding domain as a scaffold for bispecific affinity proteins

    Author : Johan Nilvebrant; Sophia Hober; Andrew Bradbury; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; albumin-binding domain; bispecific; albumin; affinity protein; phage display; staphylococcal display; orthogonal affinity purification; TNF-alpha; ErbB2; ErbB3; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. READ MORE

  5. 3. Ribosome display for selection and evolution of affibody molecules

    Author : Sebastian Grimm; Per-Åke Nygren; Mike Taussig; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; ribosome display; phage display; combinatorial protein engineering; library; murine IgG1; SATB1; Ras; Raf; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : Affinity proteins are invaluable tools in biotechnological and medical applications. This thesis is about combinatorial protein engineering principles for the generation of novel affinity proteins to purify mouse immunoglobulin, detect a potential cancer marker protein or inhibit a cell proliferation pathway. READ MORE

  6. 4. Engineering carbonic anhydrase for highly selective ester hydrolysis

    Author : Gunnar Höst; Bengt-Harald Jonsson; Lars-Göran Mårtensson; Per Berglund; Linköpings universitet; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; carbonic anhydrase; specificity; hydrolysis; rational design; protein engineering; plasmodium falciparum; Bioengineering; Bioteknik;

    Abstract : I denna avhandling presenteras arbete utfört med enzymet humant karboanhydras II (HCAII). Enzymer är en typ av proteiner som accelererar (katalyserar) kemiska reaktioner, vilket är nödvändigt för allt levande. Den naturliga funktionen för HCAII är att katalysera omvandlingen av gasen koldioxid till vätekarbonat, som är löslig i vätska. READ MORE

  7. 5. Intracellular systems for characterization and engineering of proteases and their substrates

    Author : George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. READ MORE