Search for dissertations about: "Molekylär bioteknik"

Showing result 1 - 5 of 70 swedish dissertations containing the words Molekylär bioteknik.

2. 1. Engineering carbonic anhydrase for highly selective ester hydrolysis

   Author : Gunnar Höst; Bengt-Harald Jonsson; Lars-Göran Mårtensson; Per Berglund; Linköpings universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; carbonic anhydrase; specificity; hydrolysis; rational design; protein engineering; plasmodium falciparum; Bioengineering; Bioteknik;

   Abstract : I denna avhandling presenteras arbete utfört med enzymet humant karboanhydras II (HCAII). Enzymer är en typ av proteiner som accelererar (katalyserar) kemiska reaktioner, vilket är nödvändigt för allt levande. Den naturliga funktionen för HCAII är att katalysera omvandlingen av gasen koldioxid till vätekarbonat, som är löslig i vätska. READ MORE

4. 2. Intracellular systems for characterization and engineering of proteases and their substrates

   Author : George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. READ MORE

5. 3. On bacterial formats in protein library technology

   Author : Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

   Abstract : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. READ MORE

6. 4. Chemical Synthesis of Affibody Molecules for Protein Detection and Molecular Imaging

   Author : Torun Ekblad; Amelie Eriksson Karlström; Per-Åke Nygren; Harald Kolmar; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; peptide synthesis; protein detection; molecular imaging; Bioengineering; Bioteknik;

   Abstract : Proteins are essential components in most processes in living organisms. The detection and quantification of specific proteins can be used e.g. as measures of certain physiological conditions, and are therefore of great importance. READ MORE

7. 5. Affibody molecules targeting the epidermal growth factor receptor for tumor imaging applications

   Author : Mikaela Friedman; Stefan Ståhl; Fredrik Nilsson; James S. Huston; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; affinity maturation; phage display selection; EGFR; molecular imaging; protein engineering; tumor targeting; Bioengineering; Bioteknik;

   Abstract : Tumor targeting and molecular imaging of protein markers specific for or overexpressed in tumors can add useful information in deciding upon treatment and assessing the response to treatment for a cancer patient. The epidermal growth factor receptor (EGFR) is one such tumor-associated receptor, which expression is abnormal or upregulated in various cancers and associated with a poor patient prognosis. READ MORE