Search for dissertations about: "Molekylär bioteknik"

Showing result 11 - 15 of 70 swedish dissertations containing the words Molekylär bioteknik.

2. 11. Droplet microfluidics for high throughput biological analysis

   Author : Håkan Jönsson; Helene Andersson-Svahn; Andrew Griffiths; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microfluidics; Droplet microfluidics; High throughput biology; Single cell analysis; Hydrodynamic separation; Enzyme amplification; Fluorescence polarization; Microfabrication; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : Many areas of biological research increasingly perform large-scale analyses.  In genomics the entire gene repertoire of an organism is analyzed.  Proteomics attempts to understand the function and expression patterns of all proteins in a cell or organism. READ MORE

4. 12. Characterization of Antigenic Properties and High Throughput Protein Purification

   Author : Johanna Steen; Sophia Hober; Mats Ohlin; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; high throughput; protein purification; sample preparation; antigen solubility; immunogenicity; epitope mapping; antigen design; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. READ MORE

5. 13. Structural and thermodynamical basis for molecular recognition between engineered binding proteins

   Author : Jakob Dogan; Per-Åke Nygren; John E. Ladbury; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein structure; induced fit; binding thermodynamics; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; calorimetry; Structural biochemistry; Strukturbiokemi;

   Abstract : The structural determination of interacting proteins, both as individual proteins and in their complex, complemented by thermodynamical studies are vital in order to gain in-depth insights of the phenomena leading to the highly selective protein-protein interactions characteristic of numerous life processes. This thesis describes an investigation of the structural and thermodynamical basis for molecular recognition in two different protein-protein complexes, formed between so-called affibody proteins and their respective targets. READ MORE

6. 14. An albumin-binding domain as a scaffold for bispecific affinity proteins

   Author : Johan Nilvebrant; Sophia Hober; Andrew Bradbury; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; albumin-binding domain; bispecific; albumin; affinity protein; phage display; staphylococcal display; orthogonal affinity purification; TNF-alpha; ErbB2; ErbB3; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. READ MORE

7. 15. Multiplexed antibody-based protein profiling in the pursuit of CSF biomarkers for neurodegenerative diseases

   Author : Sofia Bergström; Peter Nilsson; Anna Månberg; Markus Otto; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Protein profiling; suspension bead array; cerebrospinal fluid; biomarker discovery; proteomics; Bioteknologi; Biotechnology;

   Abstract : There is a desire for a transition from generic treatments designed for the average patient, towards more individual-based precision medicine. An increased knowledge about disease pathophysiology on a molecular level would be beneficial for this transition. READ MORE