Search for dissertations about: "Mutant Proteins"

Showing result 6 - 10 of 422 swedish dissertations containing the words Mutant Proteins.

  2. 6. From protein production to genome evolution in Escherichia coli

    Author : Susan Schlegel; Jan-Willem de Gier; Peter Neubauer; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; BL21 DE3 ; protein production; membrane proteins; secretory proteins; genome evolution; Biochemistry; biokemi;

    Abstract : The aim of my Ph.D. studies was to improve production yields of membrane- and secretory proteins in the widely used E. coli protein production strain BL21(DE3). READ MORE

  4. 7. Anticoagulant protein C--structural and functional studies

    Author : Lei Shen; Malmö Klinisk kemi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; enzymology; Proteins; Klinisk kemi; Clinical chemistry; serpins; membrane binding; factor V; protein C; protein S; Proteiner; enzymologi;

    Abstract : Anticoagulant protein C is a vitamin K-dependent factor circulating in plasma as a zymogen of a serine protease. It is converted to its active form (APC) by the thrombin-thrombomodulin complex on the surface of endothelial cells. APC inhibits the generation of thrombin by selective proteolytic inactivation procoagulant factors Va and VIIIa. READ MORE

  5. 8. EF-Tu and RNase E : Essential and Functionally Connected Proteins

    Author : Disa L. Hammarlöf; Diarmaid Hughes; Måns Ehrenberg; Gerhart Wagner; Richard H Buckingham; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; bacterial growth; translation; EF-Tu; RNase E; mRNA; RNA degradation; Molekylär cellbiologi; Molecular Cellbiology; Mikrobiologi; Microbiology;

    Abstract : The rate and accuracy of protein production is the main determinant of bacterial growth. Elongation Factor Tu (EF-Tu) provides the ribosome with aminoacylated tRNAs, and is central for its activity. In Salmonella enterica serovar Typhimurium, EF-Tu is encoded by the genes tufA and tufB. READ MORE

  6. 9. Beta-Lactamase in Haemophilus ducreyi: Purification, Characterization, and Mutagenesis

    Author : Ratana Lawung; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biokemi; Metabolism; Biochemistry; enzyme calorimeter; site-directed mutagenesis; ampicillin resistance plasmid; Haemophilus ducreyi; TEM-1 b-lactamase; metabolism; Proteins; enzymology; Proteiner; enzymologi; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : Chancroid caused by Haemophilus ducreyi, has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. Antibiotic resistances are extensively found in H. ducreyi and many of the resistances are due to the presence of resistance plasmids. READ MORE

  7. 10. Beta-Glycosidases from Hyperthermophiles as Biocatalysts

    Author : Therese Hansson; Avdelningen för klinisk kemi och farmakologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biochemical technology; Biokemisk teknik; Proteins; enzymology; Proteiner; enzymologi; Biotechnology; high temperatures; water activity; activity; selectivity; hexanol; hexyl-b-glycoside; galacto-oligosaccharides; lactose; hyperthermostable b-glycosidase; hydrolysis; optimisation; transglycosylation;

    Abstract : The thesis concerns glycosyl transfer with use of b-glycosidases, most of them from hyperthermophilic organisms. Both optimisation of the reaction conditions and protein engineering in terms of site-directed mutagenesis were used to improve b-glycosidase-catalysed reactions. READ MORE