Search for dissertations about: "NGS"

Showing result 11 - 15 of 73 swedish dissertations containing the word NGS.

2. 11. Oral microbiota in relation to host traits, environment, and dental caries

   Author : Linda Eriksson; Pernilla Lif Holgerson; Ingegerd Johansson; Anders Esberg; Peter Lingström; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Dental caries; NGS; S. mutans; sugar; sweet; diet; taste preference; adolescents; allele variation; SLC2A4; microbiota; saliva; tooth biofilm; kariologi; Cariology;

   Abstract : Background: Dental caries still appears at high prevalence worldwide. Disease distribution is skewed with more disease in socio-economically weak groups. However, also in populations considered as “low caries” there is a significant fraction with continuous disease development. READ MORE

4. 12. Extracting Genomic Variations using Selector Technology

   Author : Magnus Isaksson; Mats Nilsson; Radoje Drmanac; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Selector; Selector probe; Genetic variation; Resequencing; Targeted sequencing; copy-number variations; MLGA; Bioinformatics; Next generation sequencing; NGS; Amplified single molecule; ASM; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Genetics; Genetik; Molekylär medicin; Molecular Medicine;

   Abstract : This thesis describes the development and use of a new class of molecular tools called Selector probes, and its potential for investigations of genetic variation. The Selector technology provides multiplex amplification of targeted DNA sequences with a high specificity, and an enrichment factor in the same order of magnitude as PCR. READ MORE

5. 13. Clinical implementation of novel diagnostic biomarkers for epithelial ovarian cancer - Can we improve diagnosis?

   Author : Maria Lycke; Göteborgs universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Ovarian neoplasms; EOC; Diagnosis; protein biomarkers; HE4; CA125; Algorithms; RMI; ROMA; NGS; rare mutations; liquid biopsies; screening;

   Abstract : Background: Epithelial ovarian cancer (EOC) is the most lethal gynecologic cancer. The 5-year survival is about 30%, a consequence of failure to establish early diagnosis due to unspecific symptoms. READ MORE

6. 14. Phylogenetic Relationships, Biogeography and Species Delimitation: A case study on southern African Silene (Caryophyllaceae)

   Author : Ntwai A. Moiloa; Göteborgs universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Elisanthe; Illumina sequencing; Multispecies coalescent model; NGS; Phylogenetics; Phylogeography; species-trees; STACEY; target-capture;

   Abstract : The primary aim of this thesis was to explore the phylogenetic relationships and historical biogeography of African members of the plant genus Silene (Caryophyllaceae), with special focus on the eight native southern African taxa. Three loci (the nuclear ribosomal internal transcribed spacer region, and the rps16 and matK regions from the plastid genome) generated using traditional Sanger sequencing were used to infer the phylogenetic positions of African taxa, while sequence data from 28 low copy nuclear loci were generated using the target capture method to explore shallow phylogenetic relationships within southern African taxa. READ MORE

7. 15. The puzzle of lichen symbiosis : Pieces from Thamnolia

   Author : Ioana Onuț-Brännström; Hanna Johannesson; Leif Tibell; Anne Pringle; Uppsala universitet; []
   Keywords : Thamnolia; lichen; symbiosis; photobiont; mycobiont; phylogeography; MAT-loci; barcoding; NGS; genome; transcriptome; Ice Age; Biology with specialization in Systematics; Biologi med inriktning mot systematik;

   Abstract : Symbiosis brought important evolutionary novelties to life on Earth. Lichens, the symbiotic entities formed by fungi, photosynthetic organisms and bacteria, represent an example of a successful adaptation in surviving hostile environments. Yet many aspects of the lichen symbiosis remain unexplored. READ MORE