Search for dissertations about: "NMR Physical chemistry conformational exchange calmodulin calcium binding Fysikalisk kemi"

Found 3 swedish dissertations containing the words NMR Physical chemistry conformational exchange calmodulin calcium binding Fysikalisk kemi.

  2. 1. NMR studies on calcium-induced conformational transitions in calmodulin

    Author : Johan Evenäs; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; NMR; conformational exchange; calmodulin; calcium binding; Fysikalisk kemi;

    Abstract : Nuclear magnetic resonance (NMR) spectroscopy was used in order to investigate the relationships between structure, dynamics and calcium binding in the intracellular regulatory protein, calmodulin. Calmodulin consists of two similar domains, each binding two calcium ions with positive cooperativity. READ MORE

  4. 2. Shaking the EF-Hand. Dynamics and Ion-Binding to Calmodulin and Calbindin D9k

    Author : Anders Malmendal; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; magnesium; line shape; ion binding; EF-hand; cooperativity; conformational exchange; chemical shift; calmodulin; calcium; calbindin D9k; allostery; backbone dynamics; NMR; relaxation; Physical chemistry; Fysikalisk kemi;

    Abstract : The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. The x-ray structure of magnesium-saturated calbindin D9k shows a magnesium ion in only one of the two ion binding sites. READ MORE

  5. 3. Protein Dynamics Studied by NMR Spin Relaxation. Conformational Transitions of a Calmodulin Mutant

    Author : Patrik Lundström; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molekylär biofysik; Molecular biophysics; spin relaxation; rotating-frame; NMR; multiple-quantum; EF-hand; chemical shift; chemical exchange; calcium binding proteins; calmodulin;

    Abstract : Binding of calcium to the protein calmodulin leads to molecular reorganization that enables interaction with target peptides and activation of downstream processes. I have studied the dynamics of the calcium-loaded form of a C-terminal calmodulin mutant (E140Q-Tr2C) using NMR spin relaxation experiments. READ MORE