Search for dissertations about: "NUCLEOSIDES"

Showing result 1 - 5 of 48 swedish dissertations containing the word NUCLEOSIDES.

2. 1. Conformationally Constrained Nucleosides : Design, Synthesis, and Biochemical Evaluation of Modified Antisense Oligonucleotides

   Author : Oommen P. Varghese; Lars Engman; Jacek Stawinski; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; chemically modified oligonucleotides; azetidine; aza-ENA-T; cyanomethylene locked; free energy of activation; diaxial interactions; chair conformation; stable duplex; human serum; snake venom phosphodiesterase; antisense agents; RNase H; Organisk kemi; Chemistry; Kemi;

   Abstract : This thesis is concerned with synthesis, structure and biochemical analysis of chemically modified oligonucleotides with potential therapeutic applications. The three types of chemical modifications described here are: (a) A North-East locked 1',2'-azetidine nucleoside (b) A North locked 2',4'-cyanomethylene bridged nucleoside and (c) A 2',4'-aza-ENA-T nucleoside. READ MORE

4. 2. Conformationally Constrained Nucleosides, Nucleotides and Oligonucleotides : Design, Synthesis and Properties

   Author : Dmytro Honcharenko; Jyoti Chattopadhyaya; Piet Herdewijn; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; antisense oligonucleotides; conformationally constrained nucleosides; azetidine; aza-ENA; target affinity; RNase H; exonuclease stability; pyrene-functionalized nucleotides; fluorescence; mismatch discrimination; Bioorganisk kemi; Cell and molecular biology; Cell- och molekylärbiologi;

   Abstract : This thesis is based on six original research publications describing synthesis, structure and physicochemical and biochemical analysis of chemically modified oligonucleotides (ONs) in terms of their potential diagnostic and therapeutic applications. Synthesis of two types of bicyclic conformationally constrained nucleosides, North-East locked 1',2'-azetidine and North locked 2',4'-aza-ENA, is described. READ MORE

5. 3. Targeting the nucleotide metabolism of the mammalian pathogen Trypanosoma brucei

   Author : Munender Vodnala; Anders Hofer; Arne Holmgren; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Trypanosoma brucei; adenosine kinase; thymidine kinase; methylthioadenosine phosphorylase; mouser ribonucleotide reductase; E. coli ribonucleotide reductase; RNR; Ara-A; F-Ara-A; dNTP; NTP; doexynucleotide metabolism; nucleosides; nucleoside kinases; allosteric regulation; biologisk kemi; biological chemistry;

   Abstract : Trypanosoma brucei causes African sleeping sickness in humans and Nagana in cattle. There are no vaccines available against the disease and the current treatment is also not satisfactory because of inefficacy and numerous side effects of the used drugs.T. READ MORE

6. 4. Formation of Thiolated Nucleosides in tRNA in Salmonella enterica serovar typhimurium

   Author : Hans Lundgren; Glenn Björk; Charles Lauhon; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; : tRNA; modified nucleosides; thiolation; sulfur metabolism; Salmonella; Molecular biology; Molekylärbiologi;

   Abstract : The presence and synthesis of transfer RNA (tRNA) is highly conserved in all organisms and a lot of genetic material is dedicated to its synthesis. tRNA contains a large number of modified nucleosides and several diverse functions have been found but much about their function is still unknown. READ MORE

7. 5. Stereoelectronic effects in nucleosides and nucleotides

   Author : Christophe Thibaudeau; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi; bioorganisk kemi; Biorganic Chemistry;

   Abstract : This thesis is based on 12 original publications from 1994-98. Intramolecular stereoelectronicforces (anomeric and gauche effects), beside intermolecular forces such as hydrogen bonding,base stacking, electrostatics and hydration, have been found to be responsible for the localstructural changes of DNA and RNA and their components. READ MORE