Search for dissertations about: "Nucleic Acid Hybridization"

Showing result 1 - 5 of 15 swedish dissertations containing the words Nucleic Acid Hybridization.

  2. 1. Thermodynamics of Peptide Nucleic Acid Interactions with DNA

    Author : Tommi Ratilainen; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; thermodynamics; enthalpy-entropy compensation; ionic strength; nucleic acids; fluorescence energy transfer; PNA; calorimetry; sequence-specific binding;

    Abstract : Peptide Nucleic Acid (PNA) is a DNA mimic with the natural nucleobases attached to a charge-neutral pseudopeptide backbone. It has remarkably strong and sequence-specific binding affinity to natural nucleic acids (DNA and RNA), mainly attributed to the lack of charge in the PNA backbone. READ MORE

  4. 2. Recognition and Visualization of Nucleic Acids

    Author : Marcus Wilhelmsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; intercalation; cyanine dye; ruthenium; polarized spectroscopy; threading; groove-binding; kinetics; genetic screening; DNA; fluorescent base analogue;

    Abstract : The work presented in this Thesis focuses on the interaction between nucleic acids and small molecules, as well as binding or incorporation of reporter chromophores into nucleic acids. The results could help in studying and understanding processes that are fundamental to describe mechanisms behind a genetic disorder. READ MORE

  5. 3. Telomere analysis of normal and neoplastic hematopoietic cells : studies focusing on fluorescence in situ hybridization and flow cytometry

    Author : Magnus Hultdin; Umeå universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biomedicine; telomere; telomerase; fluorescence in situ hybridization; flow cytometry; flow-FISH; replication timing; chronic lymphocytic leukemia; immunoglobulin gene; prognosis; Biomedicin; Microbiology; Mikrobiologi; Medical Cell Biology; medicinsk cellbiologi;

    Abstract : The telomeres are specialized structures at the end of the chromosomes composed of the repeated DNA sequence (TTAGGG)n and specific proteins bound to the DNA. The telomeres protect the chromosomes from degradation and end to end fusions. READ MORE

  6. 4. The Light-up Probe

    Author : Nicke Svanvik; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

    Abstract : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. READ MORE

  7. 5. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    Author : Simon Fredriksson; Larry Gold; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Abstract : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. READ MORE