Search for dissertations about: "PC12"

Showing result 1 - 5 of 46 swedish dissertations containing the word PC12.

  2. 1. Monitoring of Cellular Dynamics with Focus on Electrochemical Detection Techniques

    Author : Arto Heiskanen; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Microfluidic system; Microchip; PC12; Saccharomyces cerevisiae; Electrochemical impedance spectroscopy; Cell adhesion; Amperometry; Exocytosis; Redox environment; Cell-based assay;

    Abstract : This thesis presents work in two main areas of focus, the development of electroanalytical techniques to monitor the dynamics of cellular processes as well as design and fabrication of microelectrode chips for electrochemical detection as well as a prototype microfluidic system to be used for cell analysis and culturing. The electrochemically monitored cellular parameters were the cellular redox environment (CRE), cell adhesion and exocytosis. READ MORE

  4. 2. Electrochemical monitoring of living cells

    Author : Christer Spegel; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; PC12; redox; electrochemistry; NAD P H; Single cell; Microchip; S. cerevisiae;

    Abstract : This thesis presents the utilization of electroanalytical methods for analysis of dynamic cellular processes such as exocytosis, intracellular redox reactions and cell surface interactions. Microchips have been designed and manufactured with the purpose of monitoring dopamine exocytosis from single or a small ensemble of PC12 cells. READ MORE

  5. 3. Experimental Studies of BMP Signalling in Neuronal Cells

    Author : Susanna Althini; Ted Ebendal; Carl-Henrik Heldin; Ingrid Strömberg; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurosciences; Bone Morphogenetic Protein BMP ; Growth Differentiation Factor 10 GDF10 ; Aktivne-receptor Like Kinase 2 ALK2 ; Tyrosine Hydroxylase TH ; Neurotrophic Factor; Neurite Outgrowth; Hippocampus; Catecholamine; PC12; Sympathetic Ganglia; Substantia Nigra SN ; Gene Targeting; Neurovetenskap; Neurology; Neurologi; Developmental Neurosciences; Medicinsk utvecklings- och neurobiologi;

    Abstract : The developing nervous system depends largely on extracellular cues to shape its complex network of neurons. Classically, neurotrophins are known to be important mediators in this process. READ MORE

  6. 4. Adenosine A2A and ATP receptors in PC12 cells

    Author : Giulia Arslan; Karolinska Institutet; Karolinska Institutet; []
    Keywords : PC12 cells; A2A receptors; ATP receptors; NGF; MAPK; Hypoxia; Cyclic AMP;

    Abstract : PC12 cells, originally isolated from a rat pheochromocytoma, are widely used to study receptor pharmacology and nerve cell differentiation. They are the neoplastic counterpart of adrenal chromaffin cells, but upon treatment with NGF they acquire a neuronal phenotype. READ MORE

  7. 5. Selective Retention of β-Carbolines and 7,12-Dimethylbenz[a]anthracene in the Brain : Role of Neuromelanin and Cytochrome P450 for Toxicity

    Author : Anna Östergren; Eva Brittebo; Nils Gunnar Lindquist; Anne-Lie Svensson; Hans Tjälve; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Toxicology; β-carboline; neuromelanin; norharman; harman; parkinsonism; Parkinson s disease; motoric impairment; behaviour; glia cells; substantia nigra; dopamine melanin; PC12 cells; ER stress; grp78; hsp90; cytochrome P450; CYP1A1; CYP1B1; blood-brain interfaces; 7; 12-dimethylbenz[a]anthracene; polycyclic aromatic hydrocarbon; endothelial cell; smooth muscle cell; bioactivation; Toxikologi; Toxicology; Toxikologi; Toxicology; toxikologi;

    Abstract : The ß-carbolines norharman and harman structurally resemble the synthetic compound 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) that is known for its ability to damage neuromelanin-containing dopaminergic neurons of the substantia nigra and thereby induce parkinsonism. MPTP is, however, not normally present in the environment whereas the ß-carbolines are present in cooked food and tobacco smoke. READ MORE