Search for dissertations about: "PCR amplification"

Showing result 1 - 5 of 105 swedish dissertations containing the words PCR amplification.

  2. 1. PCR inhibition mechanisms in forensic DNA analysis

    Author : Maja Sidstedt; Teknisk mikrobiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Abstract : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. READ MORE

  4. 2. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

    Author : Johan Banér; Sanjay Tyagi; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

    Abstract : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. READ MORE

  5. 3. PCR-based detection of microorganisms in complex biological samples

    Author : Pär-G Lantz; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. READ MORE

  6. 4. Molecular Characterization of Genomic Amplifications in Pancreatic Cancer

    Author : Markus Heidenblad; Avdelningen för klinisk genetik; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; FISH; Clinical genetics; PCR; cDNA microarray; array-based CGH; expression profiling; genomic profiling; Pancreatic cancer; gene amplification; Klinisk genetik;

    Abstract : Pancreatic cancer includes multiple histologic subtypes that show large differences in their clinical and biological characteristics. Despite this diversity, more than 85% of the neoplasms in the organ are malignant ductal adenocarcinomas, which are the focus of the present thesis. READ MORE

  7. 5. Kinetics Quality Assessment for real-time PCR

    Author : Tzachi Bar; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

    Abstract : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. READ MORE