Search for dissertations about: "PCR inhibition mechanisms"

Showing result 1 - 5 of 32 swedish dissertations containing the words PCR inhibition mechanisms.

  2. 1. PCR inhibition mechanisms in forensic DNA analysis

    Author : Maja Sidstedt; Teknisk mikrobiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Abstract : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. READ MORE

  4. 2. Leukocyte sequestration associated with inflammation : mechanisms and modulations

    Author : Kristina Nyhlén; Åke Ryrfeldt; Linköpings universitet; []
    Keywords : MEDICINE; MEDICIN;

    Abstract : Inflammationsreaktionen är kroppens första försvar mot främmande partiklar t ex bakterier. Det är en komplex reaktion som bl a karakteriseras av att vita blodkroppar dras till det inflammerade området och att vätskao i blodet lättare tar sig igenom kärlväggen till vävnaden dvs det svullnar. READ MORE

  5. 3. Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

    Author : Waleed Abu Al-Soud; Avdelningen för medicinsk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; virology; bacteriology; Microbiology; AmpliTaq Gold; Amplification facilitators; Betaine; Blood culture medium; Blood; BSA; cheese; faeces; gp32; Haemoglobin; Ions; Immunoglobulin G; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. READ MORE

  6. 4. Molecular mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosa

    Author : Shah Jalal; Karolinska Institutet; Karolinska Institutet; []
    Keywords : quinolone resistance; P. aeruginosa; PCR; DNA sequencing; mutation; NIRCA; RESA; multidrug efflux; cystic fibrosis;

    Abstract : Pseudomonas aeruginosa is an opportunistic nosocomial pathogen. In compromised human, it can cause infections in urinary tract, wound and respiratory tract such as pneumonia and progressive lung diseases. READ MORE

  7. 5. Mechanisms of p53-mediated intrinsic and extrinsic tumor suppression

    Author : Hai Li; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : p53 is a promising target for cancer therapy. However, the molecular basis of the p53 tumor suppression function remains incompletely understood. Thus, in this thesis, we focused on studies of the molecular mechanisms of p53-mediated tumor suppression. READ MORE