Search for dissertations about: "PCR inhibitors"

Showing result 1 - 5 of 59 swedish dissertations containing the words PCR inhibitors.

  1. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    University dissertation from Applied Microbiology (LTH)

    Author : Rickard Knutsson; [2001]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE

  2. 2. DNA Analysis of PCR-Inhibitory Forensic Samples

    University dissertation from Applied Microbiology (LTH)

    Author : Johannes Hedman; [2011]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amylase; statistical modelling; principal components analysis; PCR inhibitors; PCR inhibition; forensic DNA analysis; DNA polymerase blend; DNA polymerase; crime scene samples;

    Abstract : DNA evidence, linking perpetrators to crime scenes, is central to many legal proceedings. However, crime scene samples often contain extraneous substances that may interfere with the PCR-based forensic analysis, resulting in partial or negative DNA profiles. Extensive DNA purification may remove inhibitors, but involves the risk of DNA loss. READ MORE

  3. 3. PCR inhibition mechanisms in forensic DNA analysis

    University dissertation from Department of Chemistry, Lund University

    Author : Maja Sidstedt; [2019-05-24]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Abstract : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. READ MORE

  4. 4. PCR-based detection of microorganisms in complex biological samples

    University dissertation from Applied Microbiology, PO Box 124, SE-221 00 Lund, Sweden

    Author : Pär-G Lantz; [1997]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. READ MORE

  5. 5. Resistance to neuraminidase inhibitors in influenza A virus isolated from mallards

    University dissertation from Kalmar/Växjö : Linnaeus University Press

    Author : Goran Orozovic; Linnéuniversitetet.; [2011]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; influenza; mallards; neuraminidase; PCR; sequencing; mutation; oseltamivir; zanamivir; resistance; NAI; antivirals; pandemic; MEDICINE Microbiology; immunology; infectious diseases Microbiology Virology; MEDICIN Mikrobiologi; immunologi; infektionssjukdomar Mikrobiologi Virologi; NATURAL SCIENCES Biology Cell and molecular biology Molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi Molekylärbiologi; NATURAL SCIENCES Biology Other biology Bioinformatics; NATURVETENSKAP Biologi Övrig biologi Bioinformatik; Zoonotic Ecology; Zoonotisk ekologi; Virologi; Virology; Biochemistry; Biokemi;

    Abstract : Influenza A virus belongs to the Orothomyxoviridae family of viruses and is one of the most common pathogens that cause infections of the respiratory tract. The aim of this thesis was to investigate if neuraminidase inhibitor (NAI) Tamiflu® (oseltamivir, OC) and Relenza® (zanamivir, ZA) - related resistance mutations exist in the neuraminidase (NA) gene of viruses collected from wild birds. READ MORE