Search for dissertations about: "Padlock Probes"

Showing result 1 - 5 of 22 swedish dissertations containing the words Padlock Probes.

  1. 1. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Mats Nilsson; Uppsala universitet.; [1998]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. READ MORE

  2. 2. Padlock Probes and Rolling Circle Amplification New Possibilities for Sensitive Gene Detection

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Maritha Mendel-Hartvig; Uppsala universitet.; [2002]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. READ MORE

  3. 3. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Johan Banér; Uppsala universitet.; [2003]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

    Abstract : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. READ MORE

  4. 4. Genotyping RNA and DNA using padlock probes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Dan-Oscar Antson; Uppsala universitet.; [2001]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. READ MORE

  5. 5. Padlock Probe-Based Nucleic Acid Amplification Tests Point-of-care Diagnostics of Infectious Diseases

    University dissertation from Department of Biochemistry and Biophysics, Stockholm University

    Author : Sibel Ciftci; Stockholms universitet.; [2019]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Nucleic Acid Amplification; Isothermal Amplification Methods; Padlock Probes; Rolling Circle Amplification; Molecular Diagnostics; Infectious Disease Diagnostics; Virus; Point-of-Care; biokemi; Biochemistry;

    Abstract : Recent advancements in molecular biology and biotechnology have pushed the field of molecular diagnostics much further to benefit the society towards smart access for rapid and simplified health care. In this context, point-of-care (PoC) technologies that bring the inventions in diagnostics closer to bedside settings draw attention. READ MORE