Search for dissertations about: "Per-Åke Nygren"

Showing result 1 - 5 of 15 swedish dissertations containing the words Per-Åke Nygren.

  1. 1. On bacterial formats in protein library technology

    Author : Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

    Abstract : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. READ MORE

  2. 2. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Author : Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Abstract : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. READ MORE

  3. 3. Structural and thermodynamical basis for molecular recognition between engineered binding proteins

    Author : Jakob Dogan; Per-Åke Nygren; John E. Ladbury; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein structure; induced fit; binding thermodynamics; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; calorimetry; Structural biochemistry; Strukturbiokemi;

    Abstract : The structural determination of interacting proteins, both as individual proteins and in their complex, complemented by thermodynamical studies are vital in order to gain in-depth insights of the phenomena leading to the highly selective protein-protein interactions characteristic of numerous life processes. This thesis describes an investigation of the structural and thermodynamical basis for molecular recognition in two different protein-protein complexes, formed between so-called affibody proteins and their respective targets. READ MORE

  4. 4. Chemical Synthesis of Affibody Molecules for Protein Detection and Molecular Imaging

    Author : Torun Ekblad; Amelie Eriksson Karlström; Per-Åke Nygren; Harald Kolmar; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; Affibody; peptide synthesis; protein detection; molecular imaging; Bioengineering; Bioteknik;

    Abstract : Proteins are essential components in most processes in living organisms. The detection and quantification of specific proteins can be used e.g. as measures of certain physiological conditions, and are therefore of great importance. READ MORE

  5. 5. A holistic approach to understanding CAZy families through reductionist methods

    Author : Jens Eklöf; Harry Brumer; Per-Åke Nygren; Lars Arvestad; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Carbohydrate esterase family 8; XET; PME; YbhC; Biochemistry; Biokemi;

    Abstract :   In a time when the amount of biological data present in the public domain is becoming increasingly vast, the need for good classification systems has never been greater. In the field of glycoscience the necessity of a good classification for the enzymes involved in the biosynthesis, modification and degradation of polysaccharides is even more pronounced than in other fields. READ MORE