Search for dissertations about: "Periplasmic binding protein"

Showing result 1 - 5 of 19 swedish dissertations containing the words Periplasmic binding protein.

2. 1. Recombinant subunit vaccines : protein immunogens

   Author : Sissela Liljeqvist; KTH; []
   Keywords : ;

   Abstract : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. READ MORE

4. 2. Structural Studies of Binding Proteins: Investigations of Flexibility, Specificity and Stability

   Author : Ulrika Magnusson; Sherry L. Mowbray; Anthony J. Wikinson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Periplasmic binding protein; X-ray crystallography; conformational change; acidophile; protein specificity; protein structure; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Abstract : Binding proteins are present both in gram-negative and gram-positive bacteria. They are the recognition components of the ABC transport systems that transport different nutrients into the cell, and are in some cases also involved in chemotaxis. READ MORE

5. 3. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

   Author : Maria Trane; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

   Abstract : NADH:quinone oxidoreductase, or complex I, is a large, complex and poorly understood bioenergetic enzyme in the respiratory chain of living organisms. The enzyme has a conserved core structure, comprising fourteen protein subunits: Seven subunits protrude from the membrane and contain a flavin prosthetic group and eight iron-sulfur clusters that guide electrons from the oxidation of NADH towards the quinone binding site(s). READ MORE

6. 4. Structural studies of sugar binding proteins

   Author : Sanjeewani Sooriyaarachchi; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : Binding proteins, which are themselves non-enzymatic, play an important role in enzymatic reactions as well as non-enzymatic processes by providing a binding platform for the specific recognition of particular molecules. For example, periplasmic binding proteins play a vital role in nutrient uptake in Gram-negative bacteria. READ MORE

7. 5. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

   Author : Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

   Abstract : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. READ MORE