Search for dissertations about: "Plasmid"

Showing result 1 - 5 of 200 swedish dissertations containing the word Plasmid.

  2. 1. Novel Chromatography Matrices for Fast Isolation of Plasmid DNA

    Author : Peter Tiainen; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biokemisk teknik; Polymer technology; biopolymers; Polymerteknik; Biotechnology; Bioteknik; Biochemical technology; Plasmid recovery.; Composite materials; Kompositmaterial; Coating and surface treatment; Skikt och ytbehandling; Superporous agarose; Silica fibres; Plasmid purification; Anion-exchange matrices;

    Abstract : As new therapeutic areas in which genetic material is employed, i.e. gene therapy and DNA vaccination, are expanding, a substantial increase in the demand for gene vectors, e.g. READ MORE

  4. 2. CopA and CopT: The Perfect RNA Couple

    Author : Jacoba G. Slagter-Jäger; Gerhart Wagner; Wolfgang Nellen; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Microbiology; antisense RNA; plasmid; U-turn; four-way junction; RNA-RNA interaction; Hfq; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Abstract : Antisense RNAs regulate gene expression in many bacterial systems. The best characterized examples are from prokaryotic accessory elements such as phages, plasmids and transposons. Many of these antisense RNAs have been identified as plasmid copy number regulators where they regulate the replication frequency of the plasmid by negative feedback. READ MORE

  5. 3. Next generation tools for microbial cell factories

    Author : Alister James Cumming; Daniel Daley; Neil Dixon; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbial cell factories; Recombinant protein production; Bacteria; Plasmid; Genetic sensor; Inducible promoter; Genetic cassette; biokemi; Biochemistry;

    Abstract : The bacterium E. coli is a popular choice for expressing recombinant proteins. It is easy to culture and many molecular tools for are available for directing protein production. When producing recombinant proteins, high yields and good quality soluble products are desirable. READ MORE

  6. 4. The stochastic nature of intracellular control circuits

    Author : Johan Paulsson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Stochastic; mesoscopic; noise; gene; plasmid; metabolic; regulation; control; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; molekylärbiologi; Molecular Biology;

    Abstract : Chemical reactions are probabilistic by nature, causing random fluctuations inconcentrations to emerge spontaneously. In conventional (macroscopic) kinetics this internal noise is ignored under the assumption that average copy numbers are so high that fluctuations are negligible in comparison. READ MORE

  7. 5. Molecular mechanism of plasmid copy number control in Yersinia

    Author : Tifaine Héchard; Helen Wang; Javier Pizarro-Cerda; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Yersinia pseudotuberculosis; plasmid copy number; T3SS; infection; ddPCR; Mikrobiologi; Microbiology;

    Abstract : The ability of pathogenic bacteria to cause disease depends on various virulence mechanisms. The three pathogenic species of Yersinia use a type III secretion system (T3SS) to translocate effector proteins into host cells and disrupt the immune system. This T3SS is encoded on a 70kb, low-copy, virulence plasmid. READ MORE