Search for dissertations about: "Polymerase Chain Reaction PCR techniques"

Showing result 1 - 5 of 25 swedish dissertations containing the words Polymerase Chain Reaction PCR techniques.

2. 1. PCR inhibition mechanisms in forensic DNA analysis

   Author : Maja Sidstedt; Teknisk mikrobiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

   Abstract : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. READ MORE

4. 2. Detection of immunoglobulin heavy chain gene rearrangement with PCR for MRD analysis in lymphoproliferative disorders

   Author : Ulf Thunberg; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Minimal residual disease; Immunoglobulin gene rearrangement; PCR; Leukemia; Lymphoma; Myeloma; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

   Abstract : Immunoglobulin heavy chain (IGH) gene rearrangement occurs during early B-lymphocyte differentiation, assembling the different IGH gene segments to a functional gene, which can serve as a marker for study of lineage association and detection of Minimal Residual Disease (MRD) in clonal diseases deriving from B-lymphocytes or their early differentiation stages. Use of a molecular marker for the leukemic cells could help improve treatment by monitoring therapeutic efficacy, predicting relapse, and identifying very small amounts of tumour cells contaminating autografts after purging or enrichment of stem cells. READ MORE

5. 3. Detection of human papillomavirus : a study of normal cells, cervical intraepithelial neoplasia and cancer of the uterine cervix

   Author : Magnus Evander; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; HPV; PCR; General primers; Genital cancer;

   Abstract : Human papillomavirus (HPV) infections of the genital tract are now recognized to be among the most prevalent sexually transmitted diseases and also a contributing factor to some cancers of the lower genital tract of women and men. Presence of HPV in a clinical specimen is confined to detection of the HPV genome by DNA hybridization techniques. READ MORE

6. 4. Acute febrile illness in preschool children in Zanzibar - Infectious aetiologies, diagnosis and treatment

   Author : Kristina Elfving; Göteborgs universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Children; Infections; Low-income country; Tanzania; malaria; pneumonia; antibiotics; fever; diagnosis; polymerase chain reaction; preschool; influenza virus; Respiratory syncytial virus; diarrhoea; norovirus; dengue virus;

   Abstract : Background: A majority of the three million children in Africa that do not survive their fifth birthday die from infections that often start as a seemingly uncomplicated febrile illness. Primary health care workers frequently encounter febrile children with a negative malaria rapid diagnostic test (mRDT), in particular in places like Zanzibar with a considerable decline in malaria prevalence. READ MORE

7. 5. Standardization and application of quantitative PCR methods in patients with hematological malignancies

   Author : Maria Malec; Karolinska Institutet; Karolinska Institutet; []
   Keywords : Polymerase chain reaction; RQ-PCR; Minimal Residual Disease; Acute leukemia; Hodgkin s Lymphoma; cytokines;

   Abstract : Using modem combination chemotherapy regimens, it has been possible to increase the rate of complete remission (CR) in acute lymphoblastic (ALL) and acute myeloid leukemia (AML). The major reason for treatment failure in acute leukemia (AL) is the resistance of leukemic blasts to chemotherapeutic agents, which is accompanied by an inability of the immune system to eradicate residual leukemic cells surviving initial treatment. READ MORE