Search for dissertations about: "Probes"

Showing result 1 - 5 of 445 swedish dissertations containing the word Probes.

  1. 1. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    Author : Mats Nilsson; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. READ MORE

  2. 2. Probes, pronouns and binding in the Minimalist Program

    Author : Fredrik Heinat; Engelska; []
    Keywords : HUMANIORA; HUMANITIES; Grammar; probes; agree; pronouns; distributed morphology; syntax; minimalims; semantics; semiotics; Grammatik; semantik; semiotik;

    Abstract : In chapter 2, I claim that not only heads, but phrases, too, are probes. I show that there are in fact no theoretical and empirical problems involved if we abandon the stipulation that only heads may probe. In chapter 3, I explore the structure of nominal expressions. READ MORE

  3. 3. Padlock Probes and Rolling Circle Amplification : New Possibilities for Sensitive Gene Detection

    Author : Maritha Mendel-Hartvig; Pål Nyrén; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. READ MORE

  4. 4. Nuclease Activity as a Biomarker in Cancer Detection

    Author : Alien Balian; Frank Hernandez; Kajsa Uvdal; Baris Ata Borsa; Ester Boix Borrás; Linköpings universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Nucleases; Nuclease activity; Cancer detection; Diagnosis; Oligonucleotides; Nucleic acid probes; MRI-probes; Activatable probes;

    Abstract : Nucleases are a group of enzymes that cleave the phosphodiester bonds in nucleic acids. As such, nucleases act as biological scissors that exhibit a plethora of fundamental roles, in prokaryotes and eukaryotes, dependent or non-dependent on their catalytic capability. READ MORE

  5. 5. Fluorescent Probes and Protein Misfolding: Methods and Applications

    Author : Irem Nasir; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein misfolding; fluorophores; filter-trap; site-specific fluorophore labeling; amyloid-beta; microscopic rate constants; solvatochromic probes; alpha-synuclein; lipid vesicles; nanoparticles; protein adsorption;

    Abstract : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. READ MORE