Search for dissertations about: "Protein A"

Showing result 1 - 5 of 7085 swedish dissertations containing the words Protein A.

  1. 1. Protein production, characterization and structure determination in structural genomics

    University dissertation from Bioteknologi

    Author : Esmeralda A. Woestenenk; KTH.; [2004]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; NATURAL SCIENCES Biology; NATURVETENSKAP Biologi;

    Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy.The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE

  2. 2. Molecular principles of protein stability and protein-protein interactions

    University dissertation from Stockholm : KTH

    Author : Christofer Lendel; KTH.; [2005]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; TECHNOLOGY Bioengineering Structural biochemistry; TEKNIKVETENSKAP Bioteknik Strukturbiokemi;

    Abstract : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. READ MORE

  3. 3. Modulating Enzyme Functions by Semi-Rational Redesign and Chemical Modifications A Study on Mu-class Glutathione Transferases

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Malena A Norrgård; Uppsala universitet.; [2011]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein redesign; semi-rational redesign; saturation mutagenesis; iterative saturation mutagenesis; chemical modification; Cys; Cys-X scanning; enzyme evolution; promiscuous; substrate selectivity; enantioselectivity; MEDICINE Chemistry Biochemistry; MEDICIN Kemi Biokemi; Biokemi; Biochemistry;

    Abstract : Today, enzymes are extensively used for many industrial applications, this includes bulk and fine-chemical synthesis, pharmaceuticals and consumer products. Though Nature has perfected enzymes for many millions of years, they seldom reach industrial performance targets. READ MORE

  4. 4. Perplexing Protein Puzzles

    University dissertation from Department of Biophysical Chemistry, Lund University

    Author : Stina Lindman; Lund University.; Lunds universitet.; [2010]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

    Abstract : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. READ MORE

  5. 5. Membrane Protein Biogenesis in Escherichia coli : A proteomics approach

    University dissertation from Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Author : David Vikström; Stockholms universitet.; [2010]
    Keywords : SRP; YidC; SecE; Membrane protein biogenesis; protein targeting; 2D BN SDS-PAGE; biokemi; Biochemistry;

    Abstract : In the bacterium Escherichia coli all proteins are synthesized in the cytoplasm. However, at least 25% of them then need to be exported to another cellular compartment where they exert their function. E. coli has several different targeting pathways to ensure the correct localization of its proteins. READ MORE