Search for dissertations about: "Protein production"

Showing result 1 - 5 of 1058 swedish dissertations containing the words Protein production.

  1. 1. Proteome wide protein production

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Hanna Tegel; KTH.; [2013]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

    Abstract : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. READ MORE

  2. 2. Human Aquaporins: Production, Characterization and Interactions

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Jennie Sjöhamn; Göteborgs universitet.; Gothenburg University.; [2015]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; aquaporins; membrane protein; protein production; protein characterization; protein:protein interactions; structural biochemistry;

    Abstract : Membrane proteins are essential components of the cell and responsible for the communication with the outside environment and transport of molecules across the membrane. Water transport is facilitated by aquaporins, which are water selective transmembrane pores that serve to maintain cell homeostasis. READ MORE

  3. 3. Protein production, characterization and structure determination in structural genomics

    University dissertation from Bioteknologi

    Author : Esmeralda A. Woestenenk; KTH.; [2004]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; NATURAL SCIENCES Biology; NATURVETENSKAP Biologi;

    Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy.The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE

  4. 4. Recombinant subunit vaccines : protein immunogens

    University dissertation from Institutionen för biokemi och biokemisk teknologi

    Author : Sissela Liljeqvist; KTH.; [1998]
    Keywords : ;

    Abstract : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. READ MORE

  5. 5. Engineering microbial cell factories for protein production

    University dissertation from Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Author : Kiavash Mirzadeh; Stockholms universitet.; [2018]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein production; expression vector; recombinant DNA; Translation initiation region; Escherichia coli; mRNA secondary structure; Synthetic evolution; biokemi; Biochemistry;

    Abstract : Proteins are often produced using microbial cell factories for academic or industrial purposes. Protein production is however not an open-and-shut procedure. Production yields often vary in an unpredictable and context dependent manner, limiting the rational design of a straightforward production experiment. READ MORE