Search for dissertations about: "Pyrosequencing technology"

Showing result 1 - 5 of 33 swedish dissertations containing the words Pyrosequencing technology.

  1. 1. Pyrosequencing

    University dissertation from Stockholm : Bioteknologi

    Author : Samer Karamohamed; KTH.; [1999]
    Keywords : ;

    Abstract : Pyrosequencing is a method that can be used in almost allDNA sequencing applications. The method uses coupled reactionsof ATP sulfurylase and firefly luciferase to monitor theinorganic pyrophosphate (PPi) released during nucleotideincorporation onto a DNA primer. READ MORE

  2. 2. Recombinant Enzymes in Pyrosequencing Technology

    University dissertation from Stockholm : Bioteknologi

    Author : Nader Nourizad; KTH.; [2004]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

  3. 3. Approaches for analysis of mutations and genetic variations

    University dissertation from Stockholm : KTH

    Author : Afshin Ahmadian; KTH.; [2001]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LOH; microsatellite; fragment analysis; DNA sequencing; pyrosequencing; cancer; mutation; variation; SNP; allele-specific extension; apyrase; TECHNOLOGY; TEKNIKVETENSKAP;

    Abstract : Detecting mutations and genomic variations is fundamental indiagnosis, isolating disease genes, association studies,functional genomics and pharmacogenomics. The objective hasbeen to use and further develop a variety of tools andtechnologies to analyze these genetic alterations andvariations. READ MORE

  4. 4. Method development and applications of Pyrosequencing technology

    University dissertation from Stockholm : Bioteknologi

    Author : Baback Gharizadeh; KTH.; [2003]
    Keywords : apyrase; bacterial identification; dATP?S; EST sequencing; fungal identification; human papillomavirus HPV ; microbial and viral typing; multiple sequencing primer method; Pyrosequencing technology; Sequenase; single-stranded DNA-binding protein SS;

    Abstract : The ability to determine nucleic acid sequences is one ofthe most important platforms for the detailed study ofbiological systems. Pyrosequencing technology is a relativelynovel DNA sequencing technique with multifaceted uniquecharacteristics, adjustable to different strategies, formatsand instrumentations. READ MORE

  5. 5. Interrogation of Nucleic Acids by Parallel Threading

    University dissertation from Stockholm : KTH

    Author : Erik Pettersson; KTH.; [2007]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genotyping; multiplex amplification; trinucleotide threading; single nucleotide polymorphism; genotype calling; Expectation-Maximization; protein-epitope signature tag; expression profiling; Human Protein Atlas; pooled genomic DNA; Pyrosequencing; 454; allelotyping; association studies; bioinformatics; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Abstract : Advancements in the field of biotechnology are expanding the scientific horizon and a promising era is envisioned with personalized medicine for improved health. The amount of genetic data is growing at an ever-escalating pace due to the availability of novel technologies that allow massively parallel sequencing and whole-genome genotyping, that are supported by the advancements in computer science and information technologies. READ MORE