Search for dissertations about: "Pyrosequencing"

Showing result 1 - 5 of 106 swedish dissertations containing the word Pyrosequencing.

  2. 1. Pyrosequencing

    Author : Samer Karamohamed; KTH; []
    Keywords : ;

    Abstract : Pyrosequencing is a method that can be used in almost allDNA sequencing applications. The method uses coupled reactionsof ATP sulfurylase and firefly luciferase to monitor theinorganic pyrophosphate (PPi) released during nucleotideincorporation onto a DNA primer. READ MORE

  4. 2. Pyrosequencing : automation, optimization and template preparation

    Author : Tommy Nordström; KTH; []
    Keywords : alkaline phosphatase; apyrase; automation; bioluminescence; blocking oligonucleotiders;

    Abstract : .... READ MORE

  5. 3. Pyrosequencing

    Author : Mostafa Ronaghi; KTH; []
    Keywords : ;

    Abstract : .... READ MORE

  6. 4. Recombinant Enzymes in Pyrosequencing Technology

    Author : Nader Nourizad; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

  7. 5. Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification

    Author : Hanna Andréasson; Marie Allen; Ulf Gyllensten; Pål Nyrén; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; Clinical genetics; Klinisk genetik;

    Abstract : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. READ MORE