Search for dissertations about: "RNA affinity"

Showing result 1 - 5 of 58 swedish dissertations containing the words RNA affinity.

  1. 1. Conformationally Constrained Oligonucleotides for RNA Targeting

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Qing Li; Uppsala universitet.; [2012]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

    Abstract : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. READ MORE

  2. 2. Structural and Functional Studies of Wilms Tumour Protein - WT1; Novel Insights into Functionality of Zinc Finger Proteins

    University dissertation from Molecular Biophysics, Lund University

    Author : Elmar Nurmemmedov; [2007]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Naturvetenskap; Natural science; function; structure; transcription factor; cancer; affinity; kinetics; Biacore; RNA binding; DNA binding; Wilms tumour WT1 ; zinc finger;

    Abstract : The molecular process under which message in DNA is relayed onto RNA is called transcription, and it accomplished through involvement of so-called transcription factors. Zinc finger proteins are one of the most abundant classes of regulatory proteins in eukaryotic cells where they play central roles in a variety of cellular activities. READ MORE

  3. 3. Specific Precipitation of Biomolecules Using Synthetic Polymers

    University dissertation from Per-Olof Wahlund, Dept. of Biotechnology, Lund University

    Author : Per-Olof Wahlund; [2003]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Polymerteknik; RNA; plasmid DNA; DNA; polyelectrolyte complexes; synthetic polymers; precipitation; affinity precipotation; Bioteknik; Polymer technology; biopolymers;

    Abstract : Purification is a very important stage in biotechnological processes. The traditionally used precipitation techniques are generally nonspecific and thus one or more chromatographic steps are normally required. There is thus a need for new approaches to improve existing techniques. READ MORE

  4. 4. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Jharna Barman; Uppsala universitet.; [2007]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

    Abstract : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). READ MORE

  5. 5. Development of Fluorescent Nucleobase Analogues - Intrinsically labelled nucleic acids for molecular binding investigations

    University dissertation from Göteborgs universitet

    Author : Mattias Bood; Göteborgs universitet.; Gothenburg University.; [2019]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; Fluorescent nucleobase analogue; FRET; pre-microRNA; isothermal titration calorimetry; surface plasmon resonance; aminoglycoside; binding interaction;

    Abstract : This thesis focuses on the design, synthesis and utilization of fluorescent nucleobase analogues (FBAs). FBAs are an important class of compounds, used in the research of nucleic acids. The class of canonical FBAs, i.e. READ MORE