Search for dissertations about: "RNA amplification"

Showing result 16 - 20 of 65 swedish dissertations containing the words RNA amplification.

2. 16. Role of MYCN in retinoblastoma : From carcinogenesis to tumor progression

   Author : Hanzhao Zhang; Finn Hallböök; Johan Holmberg; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MYCN; retinoblastoma; RNA sequencing; cancer; avian; E2F; carcinogenesis; Developmental Neurosciences; Medicinsk utvecklings- och neurobiologi;

   Abstract : Retinoblastoma, a pediatric malignancy of the retina, is primarily driven by the bi-allelic inactivation of the RB1gene. However, a subset of cases are characterized by proficient RB1 functions but with MYCN copy number mutations, suggesting an alternative oncogenic mechanism in the absence of RB1 mutations. READ MORE

4. 17. Application of Padlock Probe Based Nucleic Acid Analysis In Situ

   Author : Sara Henriksson; Mats Nilsson; Ulf Landegren; Harvest Gu; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; rolling circle amplification; single cell gel electrophoresis assay; comet assay; Crimean Congo hemorrhagic fever virus; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

   Abstract : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. READ MORE

5. 18. Methods for Analysis of Disease Associated Genomic Sequence Variation

   Author : Lovisa Lovmar; Ann-Christine Syvänen; Pui-Yan Kwok; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; microarray; molecular medicine; single nucleotide polymorphism; whole genome amplification; breast cancer; endometrial cancer; human rotavirus; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

   Abstract : In Molecular Medicine a wide range of methods are applied to analyze the genome to find genetic predictors of human disease. Apart from predisposing disease, genetic variations may also serve as genetic markers in the search for factors underlying complex diseases. READ MORE

6. 19. Somatic Mutations in Breast Cancer Genomes : Discovery and Validation of Breast Cancer Genes

   Author : Xiang Jiao; Tobias Sjöblom; Anne-Lise Børresen-Dale; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; breast cancer; cancer gene; pathway; somatic mutation; structural alteration; sequencing; whole genome amplification; Genetics; Genetik; Medical Genetics; Medicinsk genetik;

   Abstract : Breast cancer is the most common cancer in women worldwide. However, the genetic alterations that lead to breast cancer are not fully understood. This thesis aims to identify novel genes of potential mechanistic, diagnostic or therapeutic interest in breast cancers by mutational analysis and whole-genome sequencing. READ MORE

7. 20. Ultra-sensitive measurements of magnetically labelled RCA products in a microfluidic channel using a high-Tc SQUID

   Author : Sobhan Sepehri; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; diagnostics.; high-Tc SQUID; biosensor; magnetic nanoparticle; rolling circle amplification; magnetic bioassay;

   Abstract : The development of a nucleic acid (deoxyribonucleic acid (DNA), ribonucleic acid (RNA)) bioassay based on magnetic nanoparticles (MNPs) with a high-Tc superconducting quantum interference device (SQUID) gradiometer as a magnetic readout is described. The specific binding of the MNPs to the target DNA molecules changes the MNPs size distribution and, therefore, the relaxation dynamics and is measured by magnetic ac susceptometry. READ MORE