Search for dissertations about: "RNA amplification"

Showing result 21 - 25 of 65 swedish dissertations containing the words RNA amplification.

  2. 21. In situ Sequencing : Methods for spatially-resolved transcriptome analysis

    Author : Marco Mignardi; Mats Nilsson; Xiaoliang Sunney Xie; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probes; rolling circle amplification; in situ sequencing; spatially-resolved transcriptomics; molecular diagnostics; Biochemistry; biokemi;

    Abstract : It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. READ MORE

  4. 22. Transcript profiling of small tissue samples using microarray technology

    Author : Maria Sievertzon; Joakim Lundeberg; Tom Freeman; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; Gene expression analysis; transcriptomics; microarray analysis; 3’-tag signature amplification; neural stem cells; Biologi; Biology; Biologi;

    Abstract : Through a number of biological, technological and computational achievements during the 20th century and the devoted work of hundreds of researchers the sequence of the human and other genomes are now available in public databases. The current challenge is to begin to understand the information encoded by the DNA sequence, to elucidate the functions of the proteins and RNA molecules encoded by the genes as well as how they are regulated. READ MORE

  5. 23. Padlock Probe-Based Assays for Molecular Diagnostics

    Author : Anja Mezger; Mats Nilsson; Paul Blainey; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Padlock probes; rolling circle amplification; molecular diagnostics; in situ mutation detection; Biochemistry; biokemi;

    Abstract : Treatment success often depends on the availability of accurate and reliable diagnostic assays to guide clinical practitioners in their treatment choices. An optimal test must excel in specificity and sensitivity, and depending on the application area time, low-cost and simplicity are equally important. READ MORE

  6. 24. Studies on natural antisense RNAs and microRNAs

    Author : Omid Reza Faridani; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Regulatory RNAs are found in all kingdoms of life and involved in regulation of gene expression at various steps including RNA splicing, editing, stability, modification, export, translation and chromatin remodelling. A large number of regulatory RNAs have been described recently. READ MORE

  7. 25. From single-cell transcriptomics to single-molecule counting

    Author : Saiful Islam; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : RNA-sequencing (RNA-seq) technology has been progressing so fast in the last few years and made it possible to perform transcriptome analysis at single-cell level that was even unimaginable a few years before. Nowadays, the importance of gene expression analysis at the single-cell level is increasingly appreciated for the study of complex heterogeneous tissue. READ MORE