Search for dissertations about: "Relative Quantitation"

Showing result 1 - 5 of 10 swedish dissertations containing the words Relative Quantitation.

  2. 1. Membrane protein proteomics - Novel method for membrane protein identification and quantification

    Author : Maria Bentz; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemisk teknik; mebran; steroider; Lipider; membranes; steroids; Lipids; enzymologi; Proteiner; Membrane proteins; Proteomics; Proteinase K; N-terminal labelling; Relative Quantitation; Database search; Cancer; Cytology; oncology; cancerology; Cytologi; onkologi; cancer; enzymology; Proteins; Biochemical technology;

    Abstract : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. READ MORE

  4. 2. Development and Application of Software Tools for Mass Spectrometry Imaging

    Author : Patrik Källback; Per E. Andrén; Per Svenningsson; Anna Nilsson; Benjamin Bowen; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; mass spectrometry imaging; MALDI; DESI; msIQuant; quantitation; neurotransmitters; drugs; derivatization; brain; compression; data entropy reduction;

    Abstract : Mass spectrometry imaging (MSI) has been extensively used to produce qualitative maps of distributions of proteins, peptides, lipids, neurotransmitters, small molecule pharmaceuticals and their metabolites directly in biological tissue sections. Moreover, during the last 10 years, there has been growing demand to quantify target compounds in tissue sections of various organs. READ MORE

  5. 3. Mass Spectrometry-based Neuroproteomics : Deciphering the Human Brain Proteome

    Author : Sravani Musunuri; Jonas Bergquist; Ganna Shevchenko; Jean-Charles Sanchez; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Brain; Proteomics; Mass spectrometry; Alzheimer s disease; Traumatic brain injury; Membrane proteins; Sample preparation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Abstract : Mammalian brain is challenging to study due to its heterogeneity and complexity. However, recent advances in molecular imaging, genomics and proteomics have contributed significantly to achieve insights into molecular basis of brain function and pathogenesis of neurological disorders. READ MORE

  6. 4. Development of Liquid-based Separation Techniques using Tailored Surfaces for Analysis of Biological Samples

    Author : Emilia Hardenborg; Jonas Bergquist; Marit Andersson; Alejandro Cifuentes; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; aqueous humor; biological samples; capillary electrophoresis; coatings; drugs; liquid chromatography; molecular imprinted polymers; peptides; proteins; pseudoexfoliation; Analytical chemistry; Analytisk kemi;

    Abstract : Development and improvement of analytical techniques are vital in analytical chemistry research. This thesis describes the development and use of tailored surfaces for bioanalytical applications. READ MORE

  7. 5. Antibody-Fragments: Protein Engineering, Process Development, and their Application as Affinity Ligands

    Author : Laila Sakhnini; Tillämpad biokemi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Monoclonal Antibodies; Aggregation; Biophysical Characterisation; Process Development; Affinity Adsorbents; Monoclonal Antibodies; Aggregation; Biophysical Characterisation; Process Development; Affinity Adsorbents;

    Abstract : Monoclonal antibody (mAb) fragments have become the formats of choice for both biopharmaceuticals and ligands in analytical and preparative applications. Their smaller size enables additional advantages over full-length mAbs, such as improved tissue penetration, faster blood clearance for diagnostic applications, improved capacity, and enablement of multivalent formatting. READ MORE