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Showing result 1 - 5 of 37 swedish dissertations matching the above criteria.
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1. Substrate specificity of protein kinases studied with synthetic peptides
Abstract : This thesis concentrates on the substrate specificities of protein kinases A and C (PKA, PKC). Synthetic peptides are used for that purpose, both in soluble and cellulose membrane-bound form. The, stereospecificity of both protein kinases was studied with D-amino acid containing synthetic peptides. READ MORE
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2. Substrate specificities and functional properties of human short-chain dehydrogenases / reductases
Abstract : Short-chain dehydrogenases/reductases (SDRs) constitute a universal superfamily of functionally heterogeneous proteins and participate in the metabolism of steroids, prostaglandins, retinoids, aliphatic alcohols, and xenobiotics. So far, more than 3000 members including species variants and over 60 human SDR genes are deposited in databases. READ MORE
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3. In Vitro Studies of the Substrate Specificities of Heparan Sulfate 2-O- and 6-O-sulfotransferases
Abstract : Heparan sulfate (HS), a linear negatively charged polysaccharide located at the cell surface and in the extracellular matrix, interacts with, and thereby regulates the functions of numerous proteins. HS-protein interactions depend on the fine structure of HS, especially its sulfation pattern. READ MORE
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4. galactmannan degradation by fungi and gut bacteria : structural enzymology and fine -tuned substrate specifcity
Abstract : AbstractThe degradation of plant based β-mannan polysaccharides represents one of the many challenges efficiently tackled by microorganisms living in different habitats. In this thesis, glycoside hydrolases (GHs) involved in mannan degradation from two different organisms, Aspergillus nidulans (paper I and II) and Bacteroides ovatus (paper III and IV) were studied. READ MORE
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5. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling
Abstract : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. READ MORE