Search for dissertations about: "Selenocysteine"

Showing result 1 - 5 of 24 swedish dissertations containing the word Selenocysteine.

  2. 1. Selenocysteine in proteins : properties and biotechnological use

    Author : Linda Johansson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Selenocysteine; recombinant selenoprotein production; thioredoxin reductase; enzymology; protein labeling; mite allergy; mouse model;

    Abstract : Selenocysteine (Sec), the 21st amino acid, exists in all kingdoms of life and has unique biochemical properties, such as high electrophilicity and low pKa. The highly increased reactivity of selenoenzymes compared to their sulfur-containing cysteine-dependent homologs is generally regarded as the evolutionary reason for having selenoproteins. READ MORE

  4. 2. Stretching the genetic code : incorporation of selenocysteine at specific UGA codons in recombinant proteins produced in Escherichia coli

    Author : Olle Rengby; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Selenocysteine (Sec) exists in all domains of life and represents the 21st naturally occurring amino acid. A Sec residue is co-translationally incorporated at a predefined opal (UGA) codon. UGA codons normally encode for translational stop via the protein release factor 2 (RF2). READ MORE

  5. 3. In vitro studies on the biosynthesis and reduction of ubiquinone

    Author : Tomas Nordman; Karolinska Institutet; Karolinska Institutet; []
    Keywords : The mevalonate pathway; Ubiquinone; Ubiquinol; Lipoamide dehydrogenase; Thioredoxin reductase; Glutathione reductase; Selenium;

    Abstract : This thesis concerns the role of ubiquinone, the only endogenously synthesised lipid soluble antioxidant, in the cellular defence against peroxidation of proteins and lipids. The aims of the present investigations were to study the biosynthesis of ubiquinone in two different organelle fractions, i.e. READ MORE

  6. 4. The influence of nucleotides on ribonucleotide reductase assambly in class I ribonucleotide reductase from Escherichia coli

    Author : Pernilla Larsson Birgander; Britt-Marie Sjöberg; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Molekylärbiologi;

    Abstract : The components of DNA, the deoxyribonucleotides, are produced from the components of RNA, the ribonucleotides. One single substitution is needed to convert a ribonucleotide into a deoxyribonucleotide i.e. a replacement of a hydroxyl group with a hydrogen atom. READ MORE

  7. 5. Functional characterization of cytosolic and mitochondrial thioredoxin reductases

    Author : Ivan Nalvarte; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Thioredoxin; thioredoxin reductase; reactive oxygen species; mitochondria; differentiation; redox signaling; ubiquinone; cytochrome c; selenium; selenoprotein.;

    Abstract : Mammalian thioredoxin reductases (TrxRs) are homodimeric selenoproteins belonging to the nucleotide oxidoreductase family. They contain a C-terminal penultimate selenocysteine residue, which is kept reduced by the N-terminal redox active site, CVNVGC, of the adjacent subunit. READ MORE