Search for dissertations about: "Simpson Joseph"

Found 2 swedish dissertations containing the words Simpson Joseph.

  2. 1. Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids

    Author : Ka-Weng Ieong; Måns Ehrenberg; Simpson Joseph; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; translation; efficiency-accuracy trade-off; kinetics; unnatural amino acids; Molecular Biology; Molekylärbiologi;

    Abstract : This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural amino acids?Based on the study on pH dependence of peptide bond formation, we show that the rate of the chemistry of peptidyl transfer to aminoacyl-tRNA (AA-tRNA) Gly-tRNAGly or Pro-tRNAPro limits the rate of peptide bond formation at physiological pH 7. READ MORE

  4. 2. Ribosomal translocation in real time : Method development to Applications

    Author : Chang-il Kim; Suparna Sanyal; Martin Ott; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; ribosome; EF-G; pyrene mRNA; aminoglycoside; ribosomal protein; GTP hydrolysis; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Abstract : Translational elongation is the process in which the ribosome adds one amino acid at a time to the nascent peptide chain. As the ribosome elongates the peptide chain by 14 - 20 amino acids per second and performs hundreds of such cycles per protein, ‘elongation’ is one of the most crucial steps in translation. READ MORE